| Visualization of the ischemic core on native human brain slices by potassium staining method. | |
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MedLine Citation:
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PMID: 20624426 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potassium staining method is based on the formation of potassium cobaltnitrite crystals after the treatment by sodium cobaltnitrite of brain tissue. The degree of staining correlates with the distinct potassium content of infracted and non-infarcted brain areas. The aim of the present study was to prove that potassium staining technique is a reliable method for localization of ischemic core on native whole hemisphere cryosections of stroke patients. Furthermore, potassium stained sections have been compared with appropriate postmortem MRI images of respective brains. Brains of stroke patients were removed within 24h after death and postmortem MRI scanning was performed. Horizontal cryosections of frozen brains were taken and potassium staining was performed. Using the stained whole hemisphere sections as "map" tissue sampling has been made in order to determine water and potassium content. Potassium content of infarcted samples was significantly decreased in comparison with intact regions (0.7346+/-0.2142 mg/L and 1.928+/-0.447 mg/L, respectively, p<0.01) (mean values+/-SD). Water content of affected areas (expressed in percents) has been found to be above non-infarcted regions (81.657%+/-4.07 and 72.96%+/-6.37, respectively, p<0.01). According to our results the potassium staining method of human whole hemisphere brain sections reliably differentiates focal ischemic areas from intact brain regions. In conclusion, the postmortem examination of ischemic brain could be started with making the potassium map of infarcted whole hemisphere cryosections providing guidance for targeted tissue sampling and base of comparison for further examinations. |
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Authors:
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László Csiba; Szabolcs Farkas; József Kollár; Ervin Berényi; Katalin Nagy; Dániel Bereczki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-17 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 192 ISSN: 1872-678X ISO Abbreviation: J. Neurosci. Methods Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 17-21 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Neurology, University of Debrecen Medical and Health Science Center, Móricz Zsigmond Street 22, Debrecen, H-4032, Hungary. csiba@dote.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Brain
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pathology* Brain Mapping / methods* Humans Magnetic Resonance Imaging / methods Potassium / diagnostic use*, metabolism Staining and Labeling / methods* Stroke / pathology* Water / metabolism |
| Chemical | |
Reg. No./Substance:
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7440-09-7/Potassium; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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