Document Detail


Visualization of gold and platinum nanoparticles interacting with Salmonella enteritidis and Listeria monocytogenes.
MedLine Citation:
PMID:  20856838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. The objective of the present investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-Au and nano-Pt respectively), with Salmonella Enteritidis (Gram-negative) and Listeria monocytogenes (Gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles.
METHODS: Hydrocolloids of nano-Au or nano-Pt were added to two bacteria suspensions in the following order: nano-Au + Salmonella Enteritidis; nano-Au + Listeria monocytogenes; nano-Pt + Salmonella Enteritidis; nano-Pt + Listeria monocytogenes. Samples were inspected by transmission electron microscope.
RESULTS: Visualization of morphological interaction between nano-Au and Salmonella Enteritidis and Listeria monocytogenes, showed that nano-Au were aggregated within flagella or biofilm network and did not penetrate the bacterial cell. The analysis of morphological effects of interaction of nano-Pt with bacteria revealed that nano-Pt entered cells of Listeria monocytogenes and were removed from the cells. In the case of Salmonella Enteritidis, nano-Pt were seen inside bacteria cells, probably bound to DNA and partly left bacterial cells. After washing and centrifugation, some of the nano-Pt-DNA complexes were observed within Salmonella Enteritidis.
CONCLUSION: The results indicate that the bacteria could be used as a vehicle to deliver nano-Pt to specific points in the body.
Authors:
Ewa Sawosz; André Chwalibog; Jacek Szeliga; Filip Sawosz; Marta Grodzik; Marlena Rupiewicz; Tomasz Niemiec; Katarzyna Kacprzyk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-07
Journal Detail:
Title:  International journal of nanomedicine     Volume:  5     ISSN:  1178-2013     ISO Abbreviation:  Int J Nanomedicine     Publication Date:  2010  
Date Detail:
Created Date:  2010-09-21     Completed Date:  2011-01-28     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101263847     Medline TA:  Int J Nanomedicine     Country:  New Zealand    
Other Details:
Languages:  eng     Pagination:  631-7     Citation Subset:  IM    
Affiliation:
Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
DNA, Bacterial / metabolism
Drug Delivery Systems / methods*
Gold / administration & dosage
Listeria monocytogenes* / physiology,  ultrastructure
Metal Nanoparticles / administration & dosage*,  microbiology*,  ultrastructure
Microscopy, Electron, Transmission
Nanomedicine
Platinum / administration & dosage
Salmonella enteritidis* / physiology,  ultrastructure
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 7440-06-4/Platinum; 7440-57-5/Gold
Comments/Corrections

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