Document Detail


Visualization of early embryos of the butterfly Bicyclus anynana.
MedLine Citation:
PMID:  16128409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report on the first attempts, using both light and fluorescence microscopy, to visualize the developing embryo of the butterfly Bicyclus anynana. We developed a new protocol that enabled the clear visualization of the internal egg structures in early embryogenesis (1-24 h after egg laying). Dechorionation was followed by fixation and physical dissection of the external egg structures. Observations of embryonic and extra-embryonic cells were made using a Hoechst nuclear stain that fluoresces in the blue spectrum when bound to DNA and excited with ultraviolet (UV) light under a fluorescence microscope. Preliminary data on the developmental rate of the early embryo are also presented.
Authors:
Jarod Masci; Antónia Monteiro
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  13     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-08-30     Completed Date:  2006-01-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  139-44     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, State University of New York, Buffalo, NY 14260, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bisbenzimidazole
Butterflies / embryology*
Chorion / metabolism
Histological Techniques / methods
Microscopy, Fluorescence
Zygote / ultrastructure*
Chemical
Reg. No./Substance:
23491-44-3/Bisbenzimidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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