Document Detail

Visualization of centriole-bodies using silver stain.
MedLine Citation:
PMID:  75084     Owner:  NLM     Status:  MEDLINE    
Duplicate staining of human metaphase chromosomes, first with Giemsa followed by silver, revealed the presence of two small silver-stained bodies not seen in the Giemsa stained metaphases. Similar bodies were subsequently found in the metaphases of several animal groups. The size, structure, spatial relationships to the nucleus, behavior throughout the cell cycle, and apparent universal presence of these bodies suggest that they are either centrioles or associated centriolar structures. These centriole-bodies can be seen throughout the cell cycle, even in hypotonically spread C-metaphase chromosome plates. The silver stain procedure allows enough resolution to distinguish parent and daughter centriole-bodies at interphase, thus permitting visualization of the replication, maturation and separation stages of these bodies with the light microscope.
W M Howell; T C Hsu; B M Block
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  65     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-04-17     Completed Date:  1978-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  9-20     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell Nucleus
Chromosomes, Human / ultrastructure*
Staining and Labeling*
Reg. No./Substance:

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