Document Detail


Visualization of centriole-bodies using silver stain.
MedLine Citation:
PMID:  75084     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Duplicate staining of human metaphase chromosomes, first with Giemsa followed by silver, revealed the presence of two small silver-stained bodies not seen in the Giemsa stained metaphases. Similar bodies were subsequently found in the metaphases of several animal groups. The size, structure, spatial relationships to the nucleus, behavior throughout the cell cycle, and apparent universal presence of these bodies suggest that they are either centrioles or associated centriolar structures. These centriole-bodies can be seen throughout the cell cycle, even in hypotonically spread C-metaphase chromosome plates. The silver stain procedure allows enough resolution to distinguish parent and daughter centriole-bodies at interphase, thus permitting visualization of the replication, maturation and separation stages of these bodies with the light microscope.
Authors:
W M Howell; T C Hsu; B M Block
Related Documents :
24569234 - The epigenetic sole of sex and dosage compensation.
2420534 - Immunogold labeling of metaphase cells.
2427434 - Intensity heteromorphisms of human chromosome 15p by da/dapi technique.
25381334 - Landscape of dna methylation on the x chromosome reflects cpg density, functional chrom...
15572464 - Macat--microarray chromosome analysis tool.
22747174 - Propidium iodide for making heterochromatin more evident in the c-banding technique.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  65     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-04-17     Completed Date:  1978-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  9-20     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Nucleus
Chromosomes, Human / ultrastructure*
Humans
Interphase
Metaphase
Orthoptera
Peromyscus
Silver
Staining and Labeling*
Chemical
Reg. No./Substance:
7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Estimation of the sizes of polymorphic C-bands in man by measurement of DNA content of whole chromos...
Next Document:  Lack of bilateral coherence of mu rhythm.