Document Detail


Visualization of both peroxidase and acid phosphatase isozymes from flax (Linum) on the same gels.
MedLine Citation:
PMID:  6203429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The staining of both peroxidases (PERs) and acid phosphatases (APs) following electrophoresis on acrylamide gels is described. Scanning doubly stained gels at appropriate wavelengths reveals individual isozyme bands for both enzyme systems, giving essentially identical results to those obtained from separately run and stained gels. Collection of relative mobility (Rm) data is unaffected by such tandem staining. Few complications arise from overlapping PER and AP bands, or interactions between the two staining methods and PER or AP isozymes. Even if repeat scans of a gel at different wavelengths are needed to retrieve all isozyme information, dual staining provides distinct savings in time and sample material.
Authors:
M A Fieldes; J Starobin; H Tyson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  137     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  146-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis
Electrophoresis, Polyacrylamide Gel / methods*
Guaiac
Isoenzymes / analysis
Peroxidases / analysis
Plants / enzymology*
Spectrophotometry
Staining and Labeling*
Chemical
Reg. No./Substance:
0/Isoenzymes; 9000-29-7/Guaiac; EC 1.11.1.-/Peroxidases; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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