| Visualization of FISH Probes by dual-color chromogenic in situ hybridization. | |
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MedLine Citation:
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PMID: 20093229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to 4 fluorescence in situ hybridization (FISH) probes: MYC (c-MYC), EGFR, ERBB2 (HER2), and TOP2A. The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals with an almost complete 1:1 conversion ratio. Agreement studies between the FISH assays for HER2 and TOP2A and the corresponding CISH conversion assays showed 100% concordance (kappa values of 1.0) between the CISH and FISH methods for HER2 and TOP2A status. The correlations of the gene copy number to centromere-17 ratios were similarly high, with a correlation coefficient (r) for HER2 and TOP2A of more than 0.95. Owing to the relatively small number of specimens in this study, it is important that the data are confirmed in a larger study. |
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Authors:
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Kirsten Hoff; Jan T Jørgensen; Sven Müller; Else Røngaard; Ole Rasmussen; Andreas Schønau |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 133 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-22 Completed Date: 2010-02-17 Revised Date: 2010-08-02 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 205-11 Citation Subset: AIM; IM |
Affiliation:
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Antibody Development, CMC Contrast, Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Neoplasm
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genetics Chromogenic Compounds* DNA Probes* DNA Topoisomerases, Type II, Eukaryotic / genetics DNA-Binding Proteins / genetics Gene Dosage Genes, erbB-2 Humans In Situ Hybridization, Fluorescence / methods* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/Chromogenic Compounds; 0/DNA Probes; 0/DNA-Binding Proteins; EC 5.99.1.-/DNA Topoisomerases, Type II, Eukaryotic; EC 5.99.1.3/DNA topoisomerase II alpha |
| Comments/Corrections | |
Comment In:
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Am J Clin Pathol. 2010 Aug;134(2):347-8
[PMID:
20660343
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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