Document Detail


Visual function in mice with photoreceptor degeneration and transgenic expression of channelrhodopsin 2 in ganglion cells.
MedLine Citation:
PMID:  20592196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The progression of rod and cone degeneration in retinally degenerate (rd) mice ultimately results in a complete loss of photoreceptors and blindness. The inner retinal neurons survive and several recent studies using genetically targeted, light activated channels have made these neurons intrinsically light sensitive. We crossbred a transgenic mouse line expressing channelrhodopsin2 (ChR2) under the control of the Thy1 promoter with the Pde6b(rd1) mouse, a model for retinal degeneration (rd1/rd1). Approximately 30-40% of the ganglion cells of the offspring expressed ChR2. Extracellular recordings from ChR2-expressing ganglion cells in degenerated retinas revealed their intrinsic light sensitivity which was approximately 7 log U less sensitive than the scotopic threshold and approximately 2 log U less sensitive than photopic responses of normal mice. All ChR2-expressing ganglion cells were excited at light ON. The visual performance of rd1/rd1 mice and ChR2 rd1/rd1 mice was compared. Behavioral tests showed that both mouse strains had a pupil light reflex and they were able to discriminate light fields from dark fields in the visual water task. Cortical activity maps were recorded with optical imaging. The ChR2rd1/rd1 mice did not show a better visual performance than rd1/rd1 mice. In both strains the residual vision was correlated with the density of cones surviving in the peripheral retina. The expression of ChR2 under the control of the Thy1 promoter in retinal ganglion cells does not rescue vision.
Authors:
Senthil Thyagarajan; Michiel van Wyk; Konrad Lehmann; Siegrid Löwel; Guoping Feng; Heinz Wässle
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  30     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-01     Completed Date:  2010-07-22     Revised Date:  2010-07-29    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8745-58     Citation Subset:  IM    
Affiliation:
Department of Neuroanatomy, Max-Planck-Institute for Brain Research, D-60528 Frankfurt/Main, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Disease Models, Animal
Light
Membrane Potentials / physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Photic Stimulation
Photoreceptor Cells, Vertebrate / pathology,  physiology*
Retinal Cone Photoreceptor Cells / pathology,  physiology
Retinal Degeneration / pathology,  physiopathology*
Retinal Ganglion Cells / pathology,  physiology*
Rhodopsin / genetics,  metabolism*
Vision, Ocular / physiology*
Visual Cortex / physiopathology
Visual Perception / physiology*
Chemical
Reg. No./Substance:
0/channelrhodopsin 2, mouse; 9009-81-8/Rhodopsin
Comments/Corrections
Comment In:
Nat Rev Neurosci. 2010 Aug;11(8):534

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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