Document Detail


Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix.
MedLine Citation:
PMID:  16780795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to describe the simple, rapid, and environmental-cost effective determination method for saikosaponins in complicated samples like Bupleuri radix and traditional Chinese medicines (TCM). Saikosaponin standards, extracts of Bupleuri radix and TCM, were applied to a polyethersulphone (PES) membrane and developed by acetonitrile-water (1:4, by volume). Saikosaponin a (SSa), SSc, and SSd were visually detected by an immunostaining method (called Eastern blotting technique) using a monoclonal antibody (MAb) against SSa. At least 62.5 ng of SSa, SSc, and SSd were clearly detectable individually. These coloring spot areas of saikosaponins on PES membrane were calculated by using the NIH Imaging software and three saikosaponins can be analyzed quantitatively between 62.5 ng and 1.0 microg. Saikosaponins in Bupleuri radix and TCM were determined and these results of SSa and total saikosaponin concentrations were in good agreement with those from the ELISA analysis.
Authors:
Osamu Morinaga; Shuhang Zhu; Hiroyuki Tanaka; Yukihiro Shoyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-30
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  346     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-06-26     Completed Date:  2006-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  687-92     Citation Subset:  IM    
Affiliation:
Innovation Plaza Fukuoka, Japan Science and Technology Agency, 3-8-34 Momochihama, Sawara-ku, Fukuoka 814-0001, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bupleurum / chemistry*
Chromatography
Immunoassay
Medicine, Chinese Traditional*
Molecular Structure
Oleanolic Acid / analogs & derivatives*,  analysis,  chemistry
Saponins / analysis*,  chemistry
Software
Chemical
Reg. No./Substance:
0/Saponins; 20874-52-6/saikosaponin; 508-02-1/Oleanolic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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