| Visual Arrestin 1 contributes to cone photoreceptor survival and light adaptation. | |
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MedLine Citation:
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PMID: 20019357 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To evaluate morphologic and functional contributions of Arrestin 1 (Arr1) and Arrestin 4 (Arr4) in cone photoreceptors, the authors examined the phenotypes of visual arrestin knockout mice (Arr1(-/-), Arr4(-/-), Arr1(-/-)Arr4(-/-) [Arr-DKO]) reared in darkness. METHODS: Retinal rods and cones were evaluated in wild-type (WT), Arr1(-/-), Arr4(-/-), and Arr-DKO mice using quantitative morphologic analysis, immunoblot, immunohistochemistry, TUNEL, and electroretinographic (ERG) techniques. RESULTS: Compared with either Arr4(-/-) or WT, Arr1(-/-) and Arr-DKO mice had increased apoptotic nuclei in their retinal outer nuclear layer (ONL) at postnatal day (P) 22. By P60, cone density was significantly diminished, but the ONL appeared normal. After 1 minute of background illumination, cone ERG b-wave amplitudes were similar in WT and all Arr KO mice. However, by 3 minutes and continuing through 15 minutes of light adaptation, the cone b-wave amplitudes of WT and Arr4(-/-) mice increased significantly over those of the Arr1(-/-) and Arr-DKO mice, which demonstrated no cone b-wave amplitude increase. In contrast, ERG flicker analysis after the 15-minute light adaptation period demonstrated no loss in amplitude for either Arr1(-/-) or Arr4(-/-) mice, whereas Arr-DKO had significantly lower amplitudes. When Arr1 expression was restored in Arr1(-/-) mice (+p48(Arr1-/-)), normal cone density and light-adapted ERG b-wave amplitudes were observed. CONCLUSIONS: In the adult dark-reared Arr1(-/-) and Arr-DKO mice, viable cones diminish over time. Arr1 expression is essential for cone photoreceptor survival and light adaptation, whereas either Arr1 or Arr4 is necessary for maintaining normal flicker responses. |
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Authors:
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Bruce M Brown; Teresa Ramirez; Lawrence Rife; Cheryl M Craft |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-12-17 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 51 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-05-19 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2372-80 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-9224, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Ocular
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physiology* Animals Arrestins / physiology* Cell Survival / physiology Electroretinography Fluorescent Antibody Technique, Indirect Genotype Immunoblotting In Situ Nick-End Labeling Mice Mice, Inbred C57BL Mice, Knockout Phenotype Polymerase Chain Reaction Retinal Cone Photoreceptor Cells / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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EY015851/EY/NEI NIH HHS; EY03040/EY/NEI NIH HHS; R01 EY015851-01A2/EY/NEI NIH HHS; R01 EY015851-02/EY/NEI NIH HHS; R01 EY015851-03/EY/NEI NIH HHS; R01 EY015851-04/EY/NEI NIH HHS; R01 EY015851-05/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arrestins; 0/beta-arrestin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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