Document Detail


Visible light alters yeast metabolic rhythms by inhibiting respiration.
MedLine Citation:
PMID:  24297928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exposure of cells to visible light in nature or in fluorescence microscopy often is considered to be relatively innocuous. However, using the yeast respiratory oscillation (YRO) as a sensitive measurement of metabolism, we find that non-UV visible light has a significant impact on yeast metabolism. Blue/green wavelengths of visible light shorten the period and dampen the amplitude of the YRO, which is an ultradian rhythm of cell metabolism and transcription. The wavelengths of light that have the greatest effect coincide with the peak absorption regions of cytochromes. Moreover, treating yeast with the electron transport inhibitor sodium azide has similar effects on the YRO as visible light. Because impairment of respiration by light would change several state variables believed to play vital roles in the YRO (e.g., oxygen tension and ATP levels), we tested oxygen's role in YRO stability and found that externally induced oxygen depletion can reset the phase of the oscillation, demonstrating that respiratory capacity plays a role in the oscillation's period and phase. Light-induced damage to the cytochromes also produces reactive oxygen species that up-regulate the oxidative stress response gene TRX2 that is involved in pathways that enable sustained growth in bright visible light. Therefore, visible light can modulate cellular rhythmicity and metabolism through unexpectedly photosensitive pathways.
Authors:
James Brian Robertson; Chris R Davis; Carl Hirschie Johnson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-12-02
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  110     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-25     Completed Date:  2014-02-27     Revised Date:  2014-06-24    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21130-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Color
Gene Knockout Techniques
Light*
Metabolic Networks and Pathways / radiation effects*
Oxygen Consumption / radiation effects*
Saccharomyces cerevisiae / genetics,  metabolism*,  radiation effects
Saccharomyces cerevisiae Proteins / genetics
Transcription Factors / genetics
Grant Support
ID/Acronym/Agency:
R01GM088595/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/YAP1 protein, S cerevisiae
Comments/Corrections

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