Document Detail

Viscoelastic and histologic properties in scarred rabbit vocal folds after mesenchymal stem cell injection.
MedLine Citation:
PMID:  16826069     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE/HYPOTHESIS: The aim of this study was to analyze the short-term viscoelastic and histologic properties of scarred rabbit vocal folds after injection of human mesenchymal stem cells (MSC) as well as the degree of MSC survival. Because MSCs are antiinflammatory and regenerate mesenchymal tissues, can MSC injection reduce vocal fold scarring after injury? STUDY DESIGN: Twelve vocal folds from 10 New Zealand rabbits were scarred by a localized resection and injected with human MSC or saline. Eight vocal folds were left as controls. MATERIAL AND METHODS: After 4 weeks, 10 larynges were stained for histology and evaluation of the lamina propria thickness. Collagen type I content was analyzed from six rabbits. MSC survival was analyzed by fluorescent in situ hybridization staining from three rabbits. Viscoelasticity for 10 vocal folds was analyzed in a parallel-plate rheometer. RESULTS: The rheometry on fresh-frozen samples showed decreased dynamic viscosity and lower elastic modulus (P<.01) in the scarred samples injected with MSC as compared with the untreated scarred group. Normal controls had lower dynamic viscosity and elastic modulus as compared with the scarred untreated and treated vocal folds (P<.01). Histologic analysis showed a higher content of collagen type 1 in the scarred samples as compared with the normal vocal folds and with the scarred folds treated with MSC. MSCs remained in all samples analyzed. CONCLUSIONS: The treated scarred vocal folds showed persistent MSC. Injection of scarred rabbit vocal folds with MSC rendered improved viscoelastic parameters and less signs of scarring expressed as collagen content in comparison to the untreated scarred vocal folds.
S Hertegård; J Cedervall; B Svensson; K Forsberg; F H J Maurer; D Vidovska; P Olivius; L Ahrlund-Richter; K Le Blanc
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Laryngoscope     Volume:  116     ISSN:  0023-852X     ISO Abbreviation:  Laryngoscope     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-07     Completed Date:  2006-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1248-54     Citation Subset:  IM    
Department of Logopedics and Phoniatrics, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
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MeSH Terms
Cicatrix / etiology,  pathology*,  physiopathology*
Disease Models, Animal
In Situ Hybridization, Fluorescence
Laryngeal Diseases / etiology,  pathology,  physiopathology
Mesoderm / cytology*,  transplantation
Stem Cell Transplantation / adverse effects*
Vocal Cords / pathology*,  physiopathology*

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