| Virus recovery rates for wild-type and live-attenuated vaccine strains of African horse sickness virus serotype 7 in orally infected South African Culicoides species. | |
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MedLine Citation:
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PMID: 18092976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously reported virus recovery rates from Culicoides (Avaritia) imicola Kieffer and Culicoides (Avaritia) bolitinos Meiswinkel (Diptera, Ceratopogonidae) orally infected with vaccine strain of African horse sickness virus serotype 7 (AHSV-7) were compared with results obtained from concurrently conducted oral infections with five recent AHSV-7 isolates from naturally infected horses from various localities in South Africa. Culicoides were fed sheep bloods spiked with 10(7.6) TCID(50)/mL of a live-attenuated vaccine strain AHSV-7, and with five field isolates in which virus titre in the bloodmeals ranged from 10(7.1) to 10(8.2) TCID(50)/mL). After an extrinsic incubation of 10 days at 23.5 degrees C, virus recovery rates were significantly higher in C. imicola (13.3%) and C. bolitinos (4.2%) infected with the live-attenuated virus than in midges infected with any of the field isolates. The virus recovery rates for the latter groups ranged from 0% to 9.5% for C. imicola and from 0% to 1.5% for C. bolitinos. The C. imicola population at Onderstepoort was significantly more susceptible to infection with AHSV-7 isolated at Onderstepoort than to the virus strains isolated from other localities. Results of this study suggest that tissue culture attenuation of AHSV-7 does not reduce its ability to orally infect competent Culicoides species and may even lead to enhanced replication in the vector. Furthermore, oral susceptibility in a midge population appears to vary for geographically distinct isolates of AHSV-7. |
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Authors:
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G J Venter; J T Paweska |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical and veterinary entomology Volume: 21 ISSN: 0269-283X ISO Abbreviation: Med. Vet. Entomol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-20 Completed Date: 2008-03-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8708682 Medline TA: Med Vet Entomol Country: England |
Other Details:
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Languages: eng Pagination: 377-83 Citation Subset: IM |
Affiliation:
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Department of Entomology, Agriculture Research Council-Onderstepoort Veterinary Institute, Onderstepoort, South Africa. venterg@arc.agric.za |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral African Horse Sickness / prevention & control, transmission, virology African horse sickness virus / classification, isolation & purification* Animals Ceratopogonidae / virology* Horses Insect Vectors / virology* Serotyping South Africa Vaccines, Attenuated Viral Vaccines / administration & dosage* Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Vaccines, Attenuated; 0/Viral Vaccines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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