| Virus-like particle-induced fusion from without in tissue culture cells: role of outer-layer proteins VP4 and VP7. | |
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MedLine Citation:
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PMID: 9151849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently described an assay that measures fusion from without induced in tissue culture cells by rotavirus, a nonenveloped, triple-protein-layered member of the Reoviridae family (M. M. Falconer, J. M. Gilbert, A. M. Roper, H. B. Greenberg, and J. S. Gavora, J. Virol. 69:5582-5591, 1995). The conditions required for syncytium formation are similar to those for viral penetration of the plasma membrane during the course of viral infection of host cells, as the presence of the outer-layer proteins VP4 and VP7 and the cleavage of VP4 are required. Here we present evidence that virus-like particles (VLPs) produced in Spodoptera frugiperda Sf-9 cells from recombinant baculoviruses expressing the four structural proteins of rotavirus can induce cell-cell fusion to the same extent as native rotavirus. This VLP-mediated fusion activity was dependent on trypsinization of VP4, and the strain-specific phenotype of individual VP4 molecules was retained in the syncytium assay similar to what has been seen with reassortant rotaviruses. We show that intact rotavirus and VLPs induce syncytia with cells that are permissive to rotavirus infection whereas nonpermissive cells are refractory to syncytium formation. This finding further supports our hypothesis that the syncytium assay accurately reflects very early events involved in viral infection and specifically the events related to viral entry into the cell. Our results also demonstrate that neither viral replication nor rotavirus proteins other than VP2, VP6, VP4, and VP7 are required for fusion and that both VP4 and VP7 are essential. The combination of a cell-cell fusion assay and the availability of recombinant VLPs will permit us to dissect the mechanisms of rotavirus penetration into host cells. |
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Authors:
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J M Gilbert; H B Greenberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 71 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1997 Jun |
Date Detail:
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Created Date: 1997-06-09 Completed Date: 1997-06-09 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4555-63 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305, USA. jgilbert@apollo.stanford.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Viral* Baculoviridae / genetics Capsid / physiology* Capsid Proteins* Cell Fusion* Cell Line Cercopithecus aethiops L Cells (Cell Line) Membrane Fusion Mice Receptors, Virus / metabolism Rotavirus / physiology* Spodoptera |
| Grant Support | |
ID/Acronym/Agency:
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DK38707/DK/NIDDK NIH HHS; R37AI21632/AI/NIAID NIH HHS; T32AI07328-08/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral; 0/Capsid Proteins; 0/Receptors, Virus; 0/VP4 protein, Rotavirus; 0/VP7 protein, Rotavirus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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