Document Detail

Virus-induced gene silencing of 14-3-3 genes abrogates dark repression of nitrate reductase activity in Nicotiana benthamiana.
MedLine Citation:
PMID:  17443349     Owner:  NLM     Status:  MEDLINE    
In order to study the effect of repression of 14-3-3 genes on actual activity of the nitrate reductase (NR) in Nicotiana benthamiana leaves, Nb14-3-3a gene was silenced by virus-induced gene silencing (VIGS) method using potato virus X (PVX). Expression of Nb14-3-3a as well as Nb14-3-3b genes was altogether repressed in the leaves of PVX-14-3a-infected plants. Furthermore, two-dimensional gel electrophoresis and immunoblot analysis with anti-14-3-3 antiserum suggested that the expressions of Nb14-3-3a and Nb14-3-3b proteins are accordingly repressed in PVX-14-3a-infected plants. It is well known that binding of 14-3-3 proteins to phosphorylated NR leads to substantial decrease in NR activity of leaves under darkness. Therefore, we studied the changes in NR activity in response to light/dark transitions in the leaves of PVX-14-3a-infected plants. NR activation state was kept at a high level under darkness in PVX-14-3a-infected plants, but not in PVX-green fluorescent protein (GFP)-infected and control plants. This result suggests that Nb14-3-3a and/or Nb14-3-3b proteins are indeed involved in the inactivation of NR activity under darkness in N. benthamiana.
Tatsuya Hirano; Akiko Ito; Thomas Berberich; Ryohei Terauchi; Hiromasa Saitoh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-19
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  278     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-04     Completed Date:  2007-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Faculty of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tenpaku, Nagoya, Japan.
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MeSH Terms
14-3-3 Proteins / antagonists & inhibitors,  genetics*,  metabolism
Base Sequence
Electrophoresis, Gel, Two-Dimensional
Enzyme Repression
Gene Expression Regulation, Plant / physiology*
Gene Silencing*
Molecular Sequence Data
Nitrate Reductase / biosynthesis*
Nitric Oxide / metabolism
Plant Leaves / enzymology*
Plants, Genetically Modified
Potexvirus / metabolism*
RNA, Plant
Sequence Homology, Nucleic Acid
Tobacco / enzymology*,  virology
Reg. No./Substance:
0/14-3-3 Proteins; 0/RNA, Plant; 10102-43-9/Nitric Oxide; EC Reductase

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