Document Detail


Virus-induced gene silencing of 14-3-3 genes abrogates dark repression of nitrate reductase activity in Nicotiana benthamiana.
MedLine Citation:
PMID:  17443349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to study the effect of repression of 14-3-3 genes on actual activity of the nitrate reductase (NR) in Nicotiana benthamiana leaves, Nb14-3-3a gene was silenced by virus-induced gene silencing (VIGS) method using potato virus X (PVX). Expression of Nb14-3-3a as well as Nb14-3-3b genes was altogether repressed in the leaves of PVX-14-3a-infected plants. Furthermore, two-dimensional gel electrophoresis and immunoblot analysis with anti-14-3-3 antiserum suggested that the expressions of Nb14-3-3a and Nb14-3-3b proteins are accordingly repressed in PVX-14-3a-infected plants. It is well known that binding of 14-3-3 proteins to phosphorylated NR leads to substantial decrease in NR activity of leaves under darkness. Therefore, we studied the changes in NR activity in response to light/dark transitions in the leaves of PVX-14-3a-infected plants. NR activation state was kept at a high level under darkness in PVX-14-3a-infected plants, but not in PVX-green fluorescent protein (GFP)-infected and control plants. This result suggests that Nb14-3-3a and/or Nb14-3-3b proteins are indeed involved in the inactivation of NR activity under darkness in N. benthamiana.
Authors:
Tatsuya Hirano; Akiko Ito; Thomas Berberich; Ryohei Terauchi; Hiromasa Saitoh
Related Documents :
21676339 - Staphylococcus aureus small colony variants (scvs) and their role in disease.
23818089 - The impact of gene expression analysis on evolving views on avian brain organization.
19739669 - Suppression subtractive hybridization (ssh)-based method for estimating cd-induced diff...
12836039 - Down-regulation of an anionic peroxidase in transgenic aspen and its effect on lignin c...
22458849 - Hbcipk2, a novel cbl-interacting protein kinase from halophyte hordeum brevisubulatum, ...
15293049 - Characterization of five ralf-like genes from solanum chacoense provides support for a ...
16710339 - Modular approaches to expanding the functions of living matter.
17072629 - Variations of the melanocortin-1 receptor and the glutathione-s transferase t1 and m1 g...
23677609 - A concept of eliminating nonhomologous recombination for scalable and safe aav vector g...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-19
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  278     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-04     Completed Date:  2007-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Affiliation:
Faculty of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tenpaku, Nagoya, Japan. thirano@ccmfs.meijo-u.ac.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
14-3-3 Proteins / antagonists & inhibitors,  genetics*,  metabolism
Base Sequence
Darkness
Electrophoresis, Gel, Two-Dimensional
Enzyme Repression
Gene Expression Regulation, Plant / physiology*
Gene Silencing*
Light
Molecular Sequence Data
Nitrate Reductase / biosynthesis*
Nitric Oxide / metabolism
Phylogeny
Plant Leaves / enzymology*
Plants, Genetically Modified
Potexvirus / metabolism*
RNA, Plant
Sequence Homology, Nucleic Acid
Tobacco / enzymology*,  virology
Chemical
Reg. No./Substance:
0/14-3-3 Proteins; 0/RNA, Plant; 10102-43-9/Nitric Oxide; EC 1.7.99.4/Nitrate Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Unilateral bloody nipple discharge in a two-month-old male.
Next Document:  In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.