| Virulent Newcastle disease viruselicits a strong innate immune responsein chickens. | |
| | |
MedLine Citation:
|
PMID: 21177922 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Newcastle disease virus (NDV) is an avian paramyxovirus that causes significant economic losses to the poultry industry worldwide. There is limited knowledge about the avian immune response to infection with virulent NDVs, and how this response may contribute to disease. In this study, pathogenesis and the transcriptional host response of chickens to a virulent NDV strain that rapidly causes 100% mortality was characterized. Using microarray, a strong transcriptional host response was observed in spleens at early times after infection with the induction of groups of genes involved in innate anti-viral and pro-inflammatory responses. There were multiple genes induced at 48 hours post-infection including: type I and II interferons, several cytokines and chemokines, interferon effectors and inducible nitric oxide synthase (iNOS). The increased transcription of nitric oxide synthase was confirmed by immunohistochemistry for iNOS in spleens and measured levels of nitric oxide in serum. In vitro experiments showed strong induction of the key host response genes, interferon α interferon β and interleukin 1β and interleukin 6, in splenic leukocytes at six hours post-infection in comparison to a non-virulent NDV. The robust host response to virulent NDV, in conjunction with severe pathological damage observed, is somewhat surprising considering that all NDV encode a gene, V, which functions as a suppressor of class I IFNs. Taken together, these results suggest that the host response itself may contribute to the pathogenesis of this highly virulent strain in chickens. |
| | |
Authors:
|
Cary A Rue; Leonardo Susta; Ingrid Cornax; Corrie C Brown; Darrell R Kapczynski; David L Suarez; Daniel J King; Patti J Miller; Claudio L Afonso |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-1-7 |
Journal Detail:
|
Title: The Journal of general virology Volume: - ISSN: 1465-2099 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-1-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
USDA-ARS; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increasing incidence of Streptococcus pneumoniae serotype 19A and emergence of two vaccine escape re...
Next Document: Custom-Engineered Chimeric Foot-and-Mouth Disease Vaccine Elicits Protective Immune Responses in Pig...