Document Detail

Virulence of viable but nonculturable S. Typhimurium LT2 after peracetic acid treatment.
MedLine Citation:
PMID:  16876276     Owner:  NLM     Status:  MEDLINE    
S. Typhimurium LT2 cells suspended in sterilized sewage effluent water (SEW) and in distilled water microcosms were exposed to 0, 7, 15 and 20 mg/l peracetic acid, and tested for viability and virulence. After treatment for one hour, colony forming units decreased by at least 5 log units at peracetic acid concentration of 7 mg/l. In SEW, at peracetic acid concentration of 15 mg/l, the cells were nonculturable (VNC), but retained virulence as demonstrated by invasion assays of HeLa cells. Higher concentrations (greater than or equal to 20 mg/l) resulted in bacterial death, i.e. substrate non-responsive cells. Despite morphological alterations of the bacteria after peracetic acid treatment, visualized by transmission electronic microscopy, conservation of both adhesive and invasive capacities was confirmed by scanning electron microscopy after exposure to 0-15 mg/l peracetic acid. Public health professionals need to recognize that peracetic acid-treated Salmonella is capable of modifying its physiological characteristics, including entering and recovering from the viable but nonculturable state, and may remain virulent after a stay in SEW followed by peracetic acid treatment.
A Jolivet-Gougeon; F Sauvager; M Bonnaure-Mallet; R R Colwell; M Cormier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-28
Journal Detail:
Title:  International journal of food microbiology     Volume:  112     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2006-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  147-52     Citation Subset:  IM    
Equipe Microbiologie, UPRES-EA 1254, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes I, 2 Avenue du Professeur Léon Bernard, 35043 RENNES, France.
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MeSH Terms
Adaptation, Physiological
Bacterial Adhesion / drug effects,  physiology
Colony Count, Microbial
Disinfectants / pharmacology*
Dose-Response Relationship, Drug
Hela Cells / microbiology
Microscopy, Electron, Scanning
Peracetic Acid / pharmacology*
Salmonella typhimurium / drug effects,  pathogenicity*,  physiology*,  ultrastructure
Sewage / microbiology
Water Microbiology
Reg. No./Substance:
0/Disinfectants; 0/Sewage; 79-21-0/Peracetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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