Document Detail

Virulence of swine vesicular disease virus is determined at two amino acids in capsid protein VP1 and 2A protease.
MedLine Citation:
PMID:  11597755     Owner:  NLM     Status:  MEDLINE    
To identify the genetic determinants of virulence for swine vesicular disease virus, a panel of recombinant and site-directed mutant viruses were constructed from cDNA clones of a virulent J1'73 strain and an avirulent H/3'76 strain. Initial studies mapped the genetic determinants of virulence to either or both of the two sites at nucleotide (nt) 2842, encoding VP1-132, and nt 3355, encoding 2A-20. To determine their relative importance with regard to virulence, viruses mutated at either of these two sites from the avirulent to the virulent genotype and vice versa were tested in pigs. Viruses, mutated at nt 2842 to the virulent genotype (vSVLS104MJ1) or mutated at nt 3355 to the virulent genotype (vSVLS201MJ1), slightly recovered virulence but were very weak compared with viruses with site-directed mutations at both sites (vSVLS104/201MJ1). On the other hand, viruses, mutated at nt 2842 to the avirulent genotype (vSVLS104M00) or mutated at nt 3355 to the avirulent genotype (vSVLS201M00), did not have attenuated virulence. Sequence analysis of viruses recovered from inoculated pigs revealed that reversion at nt 3355 to the virulent genotype occurred in pigs which had been inoculated with vSVLS201M00. These results suggested that both amino acids determined the virulent phenotype, but that the 2A-20 site might be the major determinant for virulence.
T Kanno; D Mackay; G Wilsden; P Kitching
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virus research     Volume:  80     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-12     Completed Date:  2001-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
Department of Exotic Diseases, National Institute of Animal Health, 6-20-1, Josuihoncho, Kodaira, 187-0022, Tokyo, Japan.
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MeSH Terms
Capsid / genetics*
Capsid Proteins*
Cysteine Endopeptidases / genetics*
Enterovirus B, Human / genetics,  isolation & purification,  pathogenicity*
Point Mutation
Recombination, Genetic
Sequence Analysis
Viral Proteins*
Reg. No./Substance:
0/Capsid Proteins; 0/Viral Proteins; 0/echovirus 9 capsid protein vp1; EC 3.4.22.-/Cysteine Endopeptidases; EC 2A, Picornavirus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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