Document Detail


Virulence of swine vesicular disease virus is determined at two amino acids in capsid protein VP1 and 2A protease.
MedLine Citation:
PMID:  11597755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To identify the genetic determinants of virulence for swine vesicular disease virus, a panel of recombinant and site-directed mutant viruses were constructed from cDNA clones of a virulent J1'73 strain and an avirulent H/3'76 strain. Initial studies mapped the genetic determinants of virulence to either or both of the two sites at nucleotide (nt) 2842, encoding VP1-132, and nt 3355, encoding 2A-20. To determine their relative importance with regard to virulence, viruses mutated at either of these two sites from the avirulent to the virulent genotype and vice versa were tested in pigs. Viruses, mutated at nt 2842 to the virulent genotype (vSVLS104MJ1) or mutated at nt 3355 to the virulent genotype (vSVLS201MJ1), slightly recovered virulence but were very weak compared with viruses with site-directed mutations at both sites (vSVLS104/201MJ1). On the other hand, viruses, mutated at nt 2842 to the avirulent genotype (vSVLS104M00) or mutated at nt 3355 to the avirulent genotype (vSVLS201M00), did not have attenuated virulence. Sequence analysis of viruses recovered from inoculated pigs revealed that reversion at nt 3355 to the virulent genotype occurred in pigs which had been inoculated with vSVLS201M00. These results suggested that both amino acids determined the virulent phenotype, but that the 2A-20 site might be the major determinant for virulence.
Authors:
T Kanno; D Mackay; G Wilsden; P Kitching
Related Documents :
11565965 - Use of narrative analysis for comparisons of the causes of fatal accidents in three cou...
12399935 - Genetic code origin: are the pathways of type glu-trna(gln) --> gln-trna(gln) molecular...
22038725 - Characterization of abl exon 7 deletion by molecular genetic and bioinformatic methods ...
2748145 - Optimization and the genetic code.
21132285 - Characterization of the major histocompatibility complex class ii dob, dpb1, and dqb1 a...
23342815 - Crohn's disease in caucasians and african americans, as defined by clinical predictors ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virus research     Volume:  80     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-12     Completed Date:  2001-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
Affiliation:
Department of Exotic Diseases, National Institute of Animal Health, 6-20-1, Josuihoncho, Kodaira, 187-0022, Tokyo, Japan. kannot@affic.go.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Capsid / genetics*
Capsid Proteins*
Cysteine Endopeptidases / genetics*
Enterovirus B, Human / genetics,  isolation & purification,  pathogenicity*
Mutation
Point Mutation
Recombination, Genetic
Sequence Analysis
Swine
Viral Proteins*
Virulence
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Viral Proteins; 0/echovirus 9 capsid protein vp1; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.29/picornain 2A, Picornavirus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Detection of equine herpesvirus type 2 (EHV-2) in horses with keratoconjunctivitis.
Next Document:  Hypervariable genes in Grapevine leafroll associated virus 1.