Document Detail


Virulence prediction of Yersinia enterocolitica by pyrolysis gas-liquid chromatography.
MedLine Citation:
PMID:  6999993     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pyrolysis gas-liquid chromatography (PGLC) was used to differentiate between HeLa cell-invasive and noninvasive strains of Yersinia enterocolitica and between Sereny-positive and -negative strains. A temperature-programmed gas-liquid chromatograph, equipped with a high-resolution Carbowax 20M coated capillary column, separated the volatiles from pyrolyzed whole cells preparations and cell wall fractions. The resulting pyrolysis elution patterns (pyrograms) were divided into 313 30-s time interval areas. The time interval areas were normalized in relation to the entire pyrogram area and were evaluated by stepwise linear discriminant analysis. The results of the PGLC-statistical analyses showed good correlation in prediction of the HeLa cell invasivity test. The technique of PGLC coupled with statistical analyses is objective, in contrast to traditional methods of determining pathogenicity of Y. enterocolitica.
Authors:
N J Stern; A W Kotula; M D Pierson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  40     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1980 Sep 
Date Detail:
Created Date:  1980-12-18     Completed Date:  1980-12-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  646-51     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques*
Cell Wall / analysis
Chromatography, Gas
Hela Cells
Humans
Yersinia / analysis,  pathogenicity*
Comments/Corrections

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