Document Detail

Virulence, immunogenicity, and protective efficacy of two recombinant Mycobacterium bovis bacillus Calmette-Guérin strains expressing the antigen ESAT-6 from Mycobacterium tuberculosis.
MedLine Citation:
PMID:  12654778     Owner:  NLM     Status:  MEDLINE    
We constructed two recombinant Mycobacterium bovis BCG (rBCG) strains expressing ESAT-6 of Mycobacterium tuberculosis, named rBCG-1 and rBCG-2. rBCG-1 contained the ESAT-6 gene linked to BCG hsp60 and expressed a fusion protein, while rBCG-2, with a secretory sequence, could secret ESAT-6 into the culture medium. There was no evidence for increased virulence of the two rBCG strains when we made a comparison between them and BCG with regard to organ bacterial loads, lung histology, and survival time. rBCG-1 induced significantly higher specific antibody titers and stronger cellular immune response than BCG, whereas rBCG-2 had immunogenicity similar to that of the parental BCG strain. Both rBCG-1 and rBCG-2 conferred marked protection against M. tuberculosis infection, yet in terms of protective efficacy, they showed no significant improvements upon conventional BCG vaccine.
Lang Bao; Wei Chen; Huidong Zhang; Xiaoying Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infection and immunity     Volume:  71     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-25     Completed Date:  2003-05-12     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1656-61     Citation Subset:  IM    
Research Unit of Infection and Immunity, West China Medical Center, Sichuan University, No. 17, 3rd Section, Ren Min Nan Road, Chengdu, Sichuan 610041, People's Republic of China.
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MeSH Terms
Antibodies, Bacterial / blood
Antigens, Bacterial / genetics,  immunology*,  metabolism
BCG Vaccine / genetics,  immunology*
Bacterial Proteins
Chaperonin 60 / genetics,  immunology,  metabolism
Flow Cytometry
Interferon-gamma / biosynthesis
Mice, Inbred BALB C
Mycobacterium bovis / genetics,  immunology,  pathogenicity*
Mycobacterium tuberculosis / immunology
Recombinant Fusion Proteins / immunology
Spleen / cytology,  immunology
Tuberculosis / immunology,  prevention & control*
Vaccines, DNA / immunology*
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/BCG Vaccine; 0/Bacterial Proteins; 0/Chaperonin 60; 0/ESAT-6 protein, Mycobacterium tuberculosis; 0/Recombinant Fusion Proteins; 0/Vaccines, DNA; 82115-62-6/Interferon-gamma

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