Document Detail


Virulence genes of Escherichia coli strains isolated from mastitic milk.
MedLine Citation:
PMID:  15458491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Escherichia coli, a Gram-negative environmental pathogen associated with bovine mastitis was isolated from the milk of 34 symptomatic cows that had been diagnosed with clinical mastitis. Eighty isolates were obtained over a 17-month period and these isolates were screened by DNA amplification for the following E. coli virulence genes: cnf1, cnf2, eaeA, eagg, einv, ltx1, stx1, stx2 and vt2e. Thirty of the bacterial isolates, obtained from 23 different cows, had toxin genes identified in their DNA. The most common virulence gene detected was stx1, with a prevalence of 31%, followed by cnf2 (7.5%), vt2e (6.25%) and eaeA (4%). The possession of different virulence genes by the bacterial isolates had no discernable impact on the health status of the cows as there was no correlation between the potential for toxin production by the E. coli isolates and the systemic clinical condition of the respective infected cows.
Authors:
A Bean; J Williamson; R T Cursons
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary medicine. B, Infectious diseases and veterinary public health     Volume:  51     ISSN:  0931-1793     ISO Abbreviation:  J. Vet. Med. B Infect. Dis. Vet. Public Health     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-10-01     Completed Date:  2004-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955260     Medline TA:  J Vet Med B Infect Dis Vet Public Health     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  285-7     Citation Subset:  IM    
Affiliation:
Molecular Genetics Laboratory, Department of Biological Sciences, University of Waikato, Hillcrest Road, Hamilton 2001, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Outer Membrane Proteins / genetics
Cattle
DNA Primers
DNA, Bacterial / analysis
Escherichia coli / genetics,  pathogenicity*
Escherichia coli Proteins / genetics*
Female
Mastitis, Bovine / microbiology*
Milk / microbiology*
Virulence / genetics
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/DNA Primers; 0/DNA, Bacterial; 0/Escherichia coli Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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