| Virological surveillance of dengue in saint martin and saint barthelemy, French west indies, using blood samples on filter paper. | |
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MedLine Citation:
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PMID: 22232467 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abstract. To strengthen active dengue surveillance in Saint Martin and Saint Barthélemy, two French Caribbean islands, we evaluated the epidemiological usefulness of collecting blood samples from NS1-positive dengue patients on filter paper to identify the dengue serotypes circulating in these regions during a 27-month period. This approach allowed dengue serotypes to be identified by reverse transcriptase-polymerase chain reaction in 90.1% of the total set of 666 samples analyzed and, in 95.5% of the samples collected during the acute phase of the disease. This prospective virological surveillance using blood samples absorbed onto filter paper, which were stored at 4°C and shipped at ambient temperature to a specialized laboratory for analysis, allowed us to avoid the logistic and financial costs associated with shipping frozen venous blood samples. This surveillance system offers a low-cost alternative for reinforcing dengue prevention in areas where specialized laboratories do not exist, notably by facilitating the early detection of potentially new dengue serotypes. |
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Authors:
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Séverine Matheus; Jean-Loup Chappert; Sylvie Cassadou; Franck Berger; Bhetty Labeau; Laetitia Bremand; Alain Winicki; Patricia Huc-Anais; Philippe Quenel; Philippe Dussart |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The American journal of tropical medicine and hygiene Volume: 86 ISSN: 1476-1645 ISO Abbreviation: Am. J. Trop. Med. Hyg. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-01-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370507 Medline TA: Am J Trop Med Hyg Country: United States |
Other Details:
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Languages: eng Pagination: 159-65 Citation Subset: AIM; IM |
Affiliation:
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Laboratoire de Virologie, Centre National de Référence des Arbovirus et Virus Influenza Région Antilles-Guyane, Institut Pasteur de la Guyane, Cayenne, French Guiana; Cellule interrégionale d'Epidémiologie Antilles-Guyane, Fort-de-France, Martinique, French West Indies; Unité d'Epidémiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire d'Analyses Médicales, Saint Barthélemy, French West Indies; Laboratoire d'Analyses Médicales, Marigot, Saint Martin, French West Indies. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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