Document Detail


Virological synapses allow HIV-1 uptake and gene expression in renal tubular epithelial cells.
MedLine Citation:
PMID:  21335514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In animal models of HIV-associated nephropathy, the expression of HIV regulatory genes in epithelial cells is sufficient to cause disease, but how the CD4-negative epithelial cells come to express HIV genes is unknown. Here, we co-cultured T cells infected with fluorescently tagged HIV with renal tubular epithelial cells and observed efficient virus transfer between these cells. The quantity of HIV transferred was much greater than that achieved by exposure to large amounts of cell-free virus and occurred without a requirement for CD4 or Env. The transfer required stable cell-cell adhesion, which could be blocked by sulfated polysaccharides or poly-anionic compounds. We found that the internalization of virus could lead to de novo synthesis of viral protein from incoming viral RNAs even in the presence of a reverse transcriptase inhibitor. These results illustrate an interaction between infected T cells and nonimmune cells, supporting the presence of virological synapses between HIV-harboring T cells and renal tubular epithelial cells, allowing viral uptake and gene expression in epithelial cells.
Authors:
Ping Chen; Benjamin K Chen; Arevik Mosoian; Thomas Hays; Michael J Ross; Paul E Klotman; Mary E Klotman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-18
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  22     ISSN:  1533-3450     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-04     Completed Date:  2011-06-16     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  496-507     Citation Subset:  IM    
Affiliation:
Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029, USA.
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MeSH Terms
Descriptor/Qualifier:
AIDS-Associated Nephropathy / etiology,  pathology,  physiopathology
CD4-Positive T-Lymphocytes / cytology,  virology
Cell Adhesion / drug effects,  physiology
Cell Communication / drug effects,  physiology*
Cell Line
Coculture Techniques
Epithelial Cells / cytology,  virology*
Gene Expression Regulation, Viral / physiology*
HIV-1 / genetics,  physiology*
Humans
Kidney Tubules, Proximal / cytology,  virology*
Polysaccharides / pharmacology
Virus Replication / physiology
Grant Support
ID/Acronym/Agency:
5R24 CA095823-04/CA/NCI NIH HHS; AI074420-02/AI/NIAID NIH HHS; P01 DK056492/DK/NIDDK NIH HHS; P01DK056492/DK/NIDDK NIH HHS; R01 AI074420/AI/NIAID NIH HHS; R01 DK078510/DK/NIDDK NIH HHS; S10RR09145-01/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Polysaccharides
Comments/Corrections
Comment In:
J Am Soc Nephrol. 2011 Mar;22(3):399-402   [PMID:  21335518 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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