Document Detail

Virological analysis in patients with human herpes virus 6-associated ocular inflammatory disorders.
MedLine Citation:
PMID:  22700707     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine whether human herpes virus 6 (HHV-6) genomic DNA and mRNA can be detected in ocular samples from patients with inflammatory disorders, and whether viral replication is involved in the development of inflammation in the eye.
METHODS: After informed consent was obtained, ocular fluid samples (aqueous humor and vitreous fluids) were collected from 350 patients with uveitis or endophthalmitis. Corneal samples were also collected from 65 patients with corneal infections. Multiplex PCR was performed to screen ocular samples from the patients for HHV-1 to HHV-8. Samples were also assayed for HHV-6 DNA using quantitative real-time PCR. Primers for nested RT-PCR were designed to detect amplification of mRNA (HHV-6 A IE1 U90).
RESULTS: PCR results indicated a total of seven patients with uveitis or endophthalmitis (7/350, 2% +) and a single patient with corneal inflammatory disease were positive for HHV-6 DNA (1/65, 1.5% +). These eight patients had high copy numbers of HHV-6 DNA, with values ranging from 4.0 × 10(3) to 5.1 × 10(6) copies/mL. Real-time PCR analysis indicated that two of these cases were HHV-6 variant A and six cases were variant B. In addition, HHV-6 mRNA was clearly detected in vitreous cells collected from one of the patients, suggesting that viral replication may occur in the eye.
CONCLUSIONS: Our results indicate that HHV-6 infection/reactivation is implicated in ocular inflammatory diseases.
Sunao Sugita; Norio Shimizu; Ken Watanabe; Manabu Ogawa; Kazuichi Maruyama; Norio Usui; Manabu Mochizuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-12
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-13     Completed Date:  2012-09-21     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4692-8     Citation Subset:  IM    
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Japan.
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MeSH Terms
Aqueous Humor / chemistry*
Corneal Diseases / virology*
DNA, Viral / analysis
Endophthalmitis / virology
Eye Infections / virology*
Herpesviridae Infections / virology*
Herpesvirus 6, Human / isolation & purification*
Middle Aged
Polymerase Chain Reaction / methods
RNA, Messenger / analysis
Uveitis / virology
Vitreous Body / chemistry*
Young Adult
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Messenger

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