| Viral induction of Zac1b through TLR3- and IRF3-dependent pathways. | |
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MedLine Citation:
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PMID: 20947170 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Zinc finger protein regulator of apoptosis and cell cycle arrest (Zac1) is a transcription factor able to induce apoptosis or cell cycle arrest through independent pathways. In spite of the important potential functions attributed to Zac1, little is known of its physiological regulation and biological function. We discovered that variant Zac1b was expressed in murine embryonic fibroblasts (MEFs) treated with polyriboinosinic polyribocytidylic acid [poly(I:C)], a synthetic double-stranded RNA. This regulation occurred mainly through Toll-Like Receptor 3 (TLR3)- and Interferon Regulatory Factor 3 (IRF3)-dependent pathways. As TLR3 and IRF3 are central activators of antiviral immunity, we hypothesized that Zac1 may be implicated in antiviral responses. In line with this notion, we observed that Zac1b was expressed in MEFs infected with Encephalomyocarditis virus (EMCV). We also observed that Zac1-deficient MEFs were less sensitive to EMCV-induced cell death than wild-type MEFs. However, Zac1 gene inactivation had no effect on the survival of mice infected with EMCV. In conclusion, this study describes for the first time a transcriptional regulation of Zac1b, induced by synthetic dsRNA and RNA viruses, the functional significance of which remains to be further investigated. |
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Authors:
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Barbara Warzée; Claire Mesnil; Didier Hober; Delphine Caloone; Dimitri Pirottin; Mutien-Marie Garigliany; Daniel Desmecht; Pierre-Vincent Drion; Laurent Journot; Pierre Lekeux; Fabrice Bureau; Christophe J Desmet |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-13 |
Journal Detail:
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Title: Molecular immunology Volume: 48 ISSN: 1872-9142 ISO Abbreviation: Mol. Immunol. Publication Date: 2010 Nov-Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: England |
Other Details:
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Languages: eng Pagination: 119-27 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Laboratory of Cellular and Molecular Physiology, GIGA-Research, University of Liege, Liege, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cardiovirus Infections / immunology* Cell Cycle Proteins / biosynthesis*, genetics, immunology Encephalomyocarditis virus Gene Expression Gene Expression Regulation / immunology* Genes, Tumor Suppressor Immunoblotting In Situ Nick-End Labeling Interferon Regulatory Factor-3 / immunology*, metabolism Mice Mice, Inbred C57BL Mice, Knockout RNA Viruses / immunology Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / immunology* Toll-Like Receptor 3 / immunology*, metabolism Transcription Factors / biosynthesis*, genetics, immunology Transfection |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Interferon Regulatory Factor-3; 0/Irf3 protein, mouse; 0/Plagl1 protein, mouse; 0/TLR3 protein, mouse; 0/Toll-Like Receptor 3; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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