Document Detail

Violaceols Function as Actin Inhibitors Inducing Cell Shape Elongation in Fibroblast Cells.
MedLine Citation:
PMID:  22878183     Owner:  NLM     Status:  Publisher    
Violaceol-I and -II were isolated from a fractionated library of marine-derived fungal metabolites. These compounds increased the calcium ion concentration inside the cell and caused F-actin aggregation in rat fibroblast 3Y1 cells within 3 h resulting in cell shape elongation. Calcium chelator BAPTA-AM (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester) inhibited violaceol-I- and -II-induced F-actin aggregation in 3Y1 cells, and hence violaceol-I- and -II act in a calcium dependent manner. Violaceol-I and -II inhibited G-actin polymerization in vitro in a dose-dependent manner and strongly associated with G-actin, at dissociation equilibrium constants of 1.44 × 10<sup>-8</sup> M and 2.52 × 10<sup>-9</sup> M respectively. Here we report the identification of a novel function of violaceol-I and -II as actin inhibitors. Violaceol-I and -II induced cell shape elongation through F-actin aggregation in 3Y1 fibroblasts. These compounds may give researchers new insights into the role of actin in tumorigenesis and lead to the development of additional anti-tumor drugs.
Yukihiro Asami; Jae-Hyuk Jang; Hyuncheol Oh; Jae Hak Sohn; Jong Won Kim; Dong Oh Moon; Osong Kwon; Makoto Kawatani; Hiroyuki Osada; Bo Yeon Kim; Jong Seog Ahn
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  -     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB).
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