Document Detail


Vimentin in a cold-water fish, the rainbow trout: highly conserved primary structure but unique assembly properties.
MedLine Citation:
PMID:  8907703     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated from a rainbow trout (Oncorhynchus mykiss) spleen cDNA library a clone coding for vimentin. The deduced amino acid sequence reveals a high degree of identity with vimentin from carp (81%), frog (71%), chick and human (73% each). Large stretches in the central alpha-helical rod are identical within all four classes of vertebrates, but in 17 residues spread over the entire rod, the two fish differ distinctly from the tetrapod species. In addition, in the more diverged non-helical head domain, a nonapeptide motif previously shown to be important for regular filament formation is conserved. Recombinant trout vimentin assembles into bona fide filaments in vitro, with a temperature optimum between 18 and 24 degrees C. Above 27 degrees C, however, filament assembly is abruptly abolished and short filaments with thickened ends as well as structures without typical intermediate filament appearance are formed. This distinguishes its assembly properties significantly from amphibian, avian and mammalian vimentin. Also in vivo, after cDNA transfection into vimentin-free mammalian epithelial cells, trout vimentin does not form typical intermediate filament arrays at 37 degrees C. At 28 degrees C, and even more pronounced at 22 degrees C, the vimentin-positive material in the transfected cells is reorganized in the perinuclear region with a partial fibrillar appearance, but typical intermediate filament arrays are not formed. Together with immunoblotting and immunolocalization data from trout tissues, where vimentin is predominantly found in glial and white blood cells, we conclude that vimentin is indeed important in its filamentous form in fish and other vertebrates, possibly fulfilling cellular functions not directly evident in gene targeting experiments carried out in mice.
Authors:
H Herrmann; M D Munick; M Brettel; B Fouquet; J Markl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  109 ( Pt 3)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-03-11     Completed Date:  1997-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  569-78     Citation Subset:  IM    
Affiliation:
Division of Cell Biology, German Cancer Research Center, Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cloning, Molecular
Conserved Sequence*
Gene Library*
Microscopy, Electron
Molecular Sequence Data
Oncorhynchus mykiss / metabolism*
Temperature
Vimentin / analysis*,  genetics
Chemical
Reg. No./Substance:
0/Vimentin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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