Document Detail


Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm.
MedLine Citation:
PMID:  21950926     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A high-speed and hardware-only algorithm using a center of mass method has been proposed for single-detector fluorescence lifetime sensing applications. This algorithm is now implemented on a field programmable gate array to provide fast lifetime estimates from a 32 × 32 low dark count 0.13 μm complementary metal-oxide-semiconductor single-photon avalanche diode (SPAD) plus time-to-digital converter array. A simple look-up table is included to enhance the lifetime resolvability range and photon economics, making it comparable to the commonly used least-square method and maximum-likelihood estimation based software. To demonstrate its performance, a widefield microscope was adapted to accommodate the SPAD array and image different test samples. Fluorescence lifetime imaging microscopy on fluorescent beads in Rhodamine 6G at a frame rate of 50 fps is also shown.
Authors:
David D-U Li; Jochen Arlt; David Tyndall; Richard Walker; Justin Richardson; David Stoppa; Edoardo Charbon; Robert K Henderson
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  16     ISSN:  1560-2281     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  -    
Other Details:
Languages:  ENG     Pagination:  096012     Citation Subset:  -    
Affiliation:
University of Sussex, Biomedical Engineering Group, Department of Engineering and Design, School of Engineering and Informatics, Brighton BN1 9QT, United KingdomUniversity of Edinburgh, Institute for Integrated Micro and Nano Systems, Joint Research Institute for Signal & Image Processing/Integrated Systems/Energy/Civil and Environmental Engineering, School of Engineering, Faraday Building, King's Buildings, Edinburgh EH9 3JL, Scotland, United KingdomUniversity of Edinburgh, SUPA, COSMIC, School of Physics and Astronomy, Joseph Black Building, King's Buildings, Edinburgh EH9 3JZ, Scotland, United KingdomSTMicroelectronics, 33 Pinkhill, Edinburgh EH12 7BF, Scotland, United KingdomFondazione Bruno Kessler, Smart Optical Sensors and Interfaces, 38122 Trento, ItalyDelft University of Technology, Faculty Electrical Engineering, Mathematics and Computer Science, Mekelweg 4, 2628CD Delft, The Netherlands.
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