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Video evaluation of the kinematics and dynamics of the beating cardiac syncytium: an alternative to the Langendorff method.
MedLine Citation:
PMID:  21786253     Owner:  NLM     Status:  Publisher    
Many important observations and discoveries in heart physiology have been made possible using the isolated heart method of Langendorff. Nevertheless, the Langendorff method has some limitations and disadvantages such as the vulnerability of the excised heart to contusions and injuries, the probability of preconditioning during instrumentation, the possibility of inducing tissue edema, and high oxidative stress, leading to the deterioration of the contractile function. To avoid these drawbacks associated with the use of a whole heart, we alternatively used beating mouse cardiac syncytia cultured in vitro in order to assess possible ergotropic, chronotropic, and inotropic effects of drugs.?To achieve this aim, we developed a method based on image processing analysis to evaluate the kinematics and the dynamics of the drug-stimulated beating syncytia starting from the video recording of their contraction movement. In this manner, in comparison with the physiological no-drug condition, we observed progressive positive ergotropic, positive chronotropic, and positive inotropic effects of 10 µM isoproterenol (ß-adrenergic agonist) and early positive ergotropic, negative chronotropic, and positive inotropic effects of 10 µM phenylephrine (a-adrenergic agonist), followed by a late phase with negative ergotropic, positive chronotropic, and negative inotropic trends.?Our method permitted a systematic study of in vitro beating syncytia, producing results consistent with previous works. Consequently, it could be used in in vitro studies of beating cardiac patches, as an alternative to Langendorff's heart in biochemical and pharmacological studies, and especially when the Langendorff technique is inapplicable (e.g., in studies about human cardiac syncytium in physiological and pathological conditions, patient-tailored therapeutics, and syncytium models derived from induced pluripotent/embryonic stem cells with genetic mutations).?Furthermore, the method could be helpful in heart tissue engineering and bioartificial heart research to "engineer the heart piece by piece." In particular, the proposed method could be useful in the identification of a suitable cell source, in the development and testing of "smart" biomaterials, and in the design and use of novel bioreactors and microperfusion systems.
Lorenzo Fassina; Antonio Di Grazia; Fabio Naro; Lucia Monaco; Maria Gabriella Cusella De Angelis; Giovanni Magenes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-17
Journal Detail:
Title:  The International journal of artificial organs     Volume:  -     ISSN:  1724-6040     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802649     Medline TA:  Int J Artif Organs     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Systems and Computer Science, University of Pavia, Pavia - Italy and Center for Tissue Engineering (C.I.T.), University of Pavia, Pavia - Italy.
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