Document Detail


Vibrio vulnificus and Vibrio parahaemolyticus in U.S. retail shell oysters: a national survey from June 1998 to July 1999.
MedLine Citation:
PMID:  11808810     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
From June 1998 to July 1999, 370 lots of oysters in the shell were sampled at 275 different establishments (71%, restaurants or oyster bars; 27%, retail seafood markets: and 2%, wholesale seafood markets) in coastal and inland markets throughout the United States. The oysters were harvested from the Gulf (49%). Pacific (14%), Mid-Atlantic (18%), and North Atlantic (11%) Coasts of the United States and from Canada (8%). Densities of Vibrio vulnificus and Vibrio parahaemolyticus were determined using a modification of the most probable number (MPN) techniques described in the Food and Drug Administration's Bacteriological Analytical Manual. DNA probes and enzyme immunoassay were used to identify suspect isolates and to determine the presence of the thermostable direct hemolysin gene associated with pathogenicity of V. parahaemolyticus. Densities of both V. vulnifcus and V. parahaemolyticus in market oysters from all harvest regions followed a seasonal distribution, with highest densities in the summer. Highest densities of both organisms were observed in oysters harvested from the Gulf Coast, where densities often exceeded 10,000 MPN/g. The majority (78%) of lots harvested in the North Atlantic, Pacific, and Canadian Coasts had V. vulnificus densities below the detectable level of 0.2 MPN/g; none exceeded 100 MPN/g. V. parahaemolyticus densities were greater than those of V. vulnificus in lots from these same areas, with some lots exceeding 1,000 MPN/g for V. parahaemolyticus. Some lots from the Mid-Atlantic states exceeded 10,000 MPN/g for both V. vulnificus and V. parahaemolyicus. Overall, there was a significant correlation between V. vulificus and V. parahaemolyticus densities (r = 0.72, n = 202, P < 0.0001), but neither density correlated with salinity. Storage time significantly affected the V. vulnificus (10% decrease per day) and V. parahaemolyticus (7% decrease per day) densities in market oysters. The thermostable direct hemolysin gene associated with V parahaemolyticus virulence was detected in 9 of 3,429 (0.3%) V. parahaemolyticus cultures and in 8 of 198 (4.0%) lots of oysters. These data can be used to estimate the exposure of raw oyster consumers to V. vulnificus and V. parahaemolyticus.
Authors:
David W Cook; Paul Oleary; Jeff C Hunsucker; Edna M Sloan; John C Bowers; Robert J Blodgett; Angelo Depaola
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  65     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-25     Completed Date:  2002-02-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-87     Citation Subset:  IM    
Affiliation:
Gulf Coast Seafood Laboratory, Food and Drug Administration, Dauphin Island, Alabama 36528, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriological Techniques / methods*
Colony Count, Microbial
Food Contamination
Food Handling / methods*
Food Microbiology
Humans
Ostreidae / microbiology*
Restaurants
Seafood / microbiology
Seasons
Time Factors
United States
Vibrio / genetics,  isolation & purification*
Vibrio parahaemolyticus / genetics,  isolation & purification*
Water Microbiology
Comments/Corrections
Erratum In:
J Food Prot 2002 Mar;65(3):445

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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