| Vibrio vulnificus-induced death of Jurkat T-cells requires activation of p38 mitogen-activated protein kinase by NADPH oxidase-derived reactive oxygen species. | |
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MedLine Citation:
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PMID: 18571150 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vibrio vulnificus, a pathogenic bacterium causing primary septicemia, exhibited cytotoxicity towards Jurkat cells of T-lymphocytes through intracellular reactive oxygen species (ROS) production. Pretreatment of Jurkat T-cells with diphenyleneiodonium chloride (DPI) abolished V. vulnificus-induced ROS generation and bacterial ability to cause cell death. Jurkat T-cells expressing dominant-negative protein of Rac subunit of NADPH oxidase (NOX) did not show increased ROS production and cell death by V. vulnificus. Vibrio vulnificus also triggered phosphorylation of mitogen-activated protein kinases (MAPKs) including p38 and ERK1/2 in Jurkat T-cells. Experiments using inhibitors or small interfering RNAs for each MAPK showed that both MAPKs are involved in V. vulnificus-induced cell death. DPI only blocked the phosphorylation of p38 MAPK in Jurkat T-cells exposed by V. vulnificus. This study demonstrates that V. vulnificus induces death of Jurkat T-cells via ROS-dependent activation of p38 MAPK, and that NOX plays a major role in ROS generation in V. vulnificus-exposed cells. |
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Authors:
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Woo Hyang Kim; Sung Young Goo; Myeong Heon Shin; Se-Jin Chun; Heuiran Lee; Kyu-Ho Lee; Soon-Jung Park |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-20 |
Journal Detail:
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Title: Cellular immunology Volume: 253 ISSN: 1090-2163 ISO Abbreviation: Cell. Immunol. Publication Date: 2008 May-Jun |
Date Detail:
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Created Date: 2008-09-18 Completed Date: 2008-10-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 1246405 Medline TA: Cell Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 81-91 Citation Subset: IM |
Affiliation:
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Department of Environmental Medical Biology and Institute of Tropical Medicine, The Brain Korea 21 Project, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Death* Enzyme Activation Enzyme Inhibitors / metabolism Humans Jurkat Cells Mitogen-Activated Protein Kinase 1 / genetics, metabolism Mitogen-Activated Protein Kinase 3 / genetics, metabolism NADPH Oxidase / metabolism* Nitric Oxide / metabolism Nitric Oxide Synthase Type II / metabolism Onium Compounds / metabolism RNA Interference Reactive Oxygen Species / metabolism* T-Lymphocytes / enzymology, microbiology, physiology* Vibrio vulnificus / pathogenicity* p38 Mitogen-Activated Protein Kinases / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Onium Compounds; 0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide; 244-54-2/diphenyleneiodonium; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.6.3.1/NADPH Oxidase; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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