Document Detail

Vibrio vulnificus cytolysin induces apoptosis in HUVEC, SGC-7901 and SMMC-7721 cells via caspase-9/3-dependent pathway.
MedLine Citation:
PMID:  19167479     Owner:  NLM     Status:  MEDLINE    
Vibrio vulnificus cytolysin (VVC) is known to be a pore-forming toxin which shows cytotoxicity for mammalian cells in culture and induces apoptosis in endothelial cells. In order to determine whether VVC induces apoptosis in vascular endothelial cells and tumor cells, the cytotoxicity induced by recombinant VVC (rVVC) and its potential mechanism in HUVEC, SGC-7901 and SMMC-7721 cells were investigated. Our study demonstrated that rVVC induced the release of intracellular K(+) from all the target cells, yet lactate dehydrogenase was not released by rVVC. It indicates that osmotic lysis might not contribute to the cytolysin-induced cytotoxicity. The study also demonstrated that rVVC induced apoptosis in HUVEC, SGC-7901 and SMMC-7721 cells in time- and dosage-dependent manners, which was associated with the activation of caspase-9 and -3, but not caspase-8. During the apoptotic process of the target cells, rVVC labeled with FITC was monitored to attach initially to the surface of the cells and entered the cytoplasma subsequently. These findings suggest that VVC may be not only a pore-forming toxin, but also a transmembrane toxin with powerful ability to induce apoptosis in human vascular endothelial cells and tumor cells.
Jin-fang Zhao; Ai-hua Sun; Ping Ruan; Xu-hong Zhao; Miao-quan Lu; Jie Yan
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Publication Detail:
Type:  Journal Article     Date:  2009-01-06
Journal Detail:
Title:  Microbial pathogenesis     Volume:  46     ISSN:  1096-1208     ISO Abbreviation:  Microb. Pathog.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-06-04     Completed Date:  2009-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  England    
Other Details:
Languages:  eng     Pagination:  194-200     Citation Subset:  IM    
Basic Medical Microbiology Division, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, PR China.
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MeSH Terms
Caspase 3 / metabolism*
Caspase 8 / metabolism
Caspase 9 / metabolism*
Cell Line, Tumor
Cell Membrane / chemistry
Cell Survival / drug effects
Cells, Cultured
Cytoplasm / chemistry
Endothelial Cells / drug effects*
Epithelial Cells / drug effects
Hepatocytes / drug effects
L-Lactate Dehydrogenase / metabolism
Perforin / toxicity*
Potassium / metabolism
Vibrio vulnificus / pathogenicity*
Reg. No./Substance:
126465-35-8/Perforin; 7440-09-7/Potassium; EC Dehydrogenase; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9

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