Document Detail


Vibrio parahaemolyticus ScrC modulates cyclic dimeric GMP regulation of gene expression relevant to growth on surfaces.
MedLine Citation:
PMID:  17993539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Vibrio parahaemolyticus, scrC participates in controlling the decision to be a highly mobile swarmer cell or a more adhesive, biofilm-proficient cell type. scrC mutants display decreased swarming motility over surfaces and enhanced capsular polysaccharide production. ScrC is a cytoplasmic membrane protein that contains both GGDEF and EAL conserved protein domains. These domains have been shown in many organisms to respectively control the formation and degradation of the small signaling nucleotide cyclic dimeric GMP (c-di-GMP). The scrC gene is part of the three-gene scrABC operon. Here we report that this operon influences the cellular nucleotide pool and that c-di-GMP levels inversely modulate lateral flagellar and capsular polysaccharide gene expression. High concentrations of this nucleotide prevent swarming and promote adhesiveness. Further, we demonstrate that ScrC has intrinsic diguanylate cyclase and phosphodiesterase activities, and these activities are controlled by ScrAB. Specifically, ScrC acts to form c-di-GMP in the absence of ScrA and ScrB; whereas ScrC acts to degrade c-di-GMP in the presence of ScrA and ScrB. The scrABC operon is specifically induced by growth on a surface, and the analysis of mutant phenotypes supports a model in which the phosphodiesterase activity of ScrC plays a dominant role during surface translocation and in biofilms.
Authors:
Rosana B R Ferreira; Luis Caetano M Antunes; E Peter Greenberg; Linda L McCarter
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-11-09
Journal Detail:
Title:  Journal of bacteriology     Volume:  190     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-18     Completed Date:  2008-02-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  851-60     Citation Subset:  IM    
Affiliation:
Department of Microbiology, The University of Iowa, Iowa City, Iowa 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
Bacterial Capsules / genetics,  metabolism
Bacterial Proteins / chemistry,  genetics,  metabolism*
Cyclic GMP / metabolism,  pharmacology*
Dimerization
Flagella / genetics,  metabolism
Gene Expression Regulation, Bacterial*
Membrane Proteins / chemistry,  genetics,  metabolism*
Mutation
Operon
Phosphoric Diester Hydrolases / chemistry,  genetics,  metabolism
Phosphorus-Oxygen Lyases / chemistry,  genetics,  metabolism
Vibrio parahaemolyticus / genetics,  growth & development*,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Capsules; 0/Bacterial Proteins; 0/Membrane Proteins; 7665-99-8/Cyclic GMP; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 4.6.-/Phosphorus-Oxygen Lyases; EC 4.6.1.-/diguanylate cyclase
Comments/Corrections
Comment In:
J Bacteriol. 2008 Feb;190(3):781-3   [PMID:  18065536 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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