Document Detail


Vibrio parahaemolyticus infection induces modulation of IL-8 secretion through dual pathway via VP1680 in Caco-2 cells.
MedLine Citation:
PMID:  21177635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Vibrio parahaemolyticus causes acute gastroenteritis and inflammations in humans. A variety of pathogenic bacteria can stimulate mitogen-activated protein kinases (MAPKs) in host cells. Phosphorylation of MAPKs leads to production of interleukin (IL)- 8 and subsequently causes inflammations. Thus, MAPK cascades were strong candidates for the main signaling pathway of V. parahaemolyticus-induced acute inflammation.
METHODS: To determine whether the signaling pathway on V. parahaemolyticus infection induces inflammation, we analyzed the secretion level of IL-8 and phosphorylation of MAPKs by use of intestinal epithelial Caco-2 cells.
RESULTS: V. parahaemolyticus infection of Caco-2 cells activated extracellular signal-regulated kinase (ERK) 1/2 and p38 MAPK signal pathways, leading to IL-8 secretion, whereas MAPK inhibitors, UO126 or SB203580, suppressed IL-8 secretion. A strain carrying a deletion of VP1680, a type three secretion system 1 (T3SS1) effector protein, failed to activate phosphorylation of ERK1/2 and p38 MAPK and secretion of IL-8. ERK1/2 pathway inhibitor, UO126, failed IL-8 promoter activity, whereas p38 MAPK inhibitor, SB203580, decreased the stabilization of IL-8 messenger RNA following V. parahaemolyticus infection.
CONCLUSIONS: We showed that V. parahaemolyticus infection of Caco-2 cells results in the secretion of IL-8, and that VP1680 plays a pivotal role in manipulating host cell signaling and is responsible for triggering IL-8 secretion.
Authors:
Takaaki Shimohata; Masayuki Nakano; Xin Lian; Tomomi Shigeyama; Hitomi Iba; Akiko Hamamoto; Masaki Yoshida; Nagakatsu Harada; Hironori Yamamoto; Masayuki Yamato; Kazuaki Mawatari; Toshiaki Tamaki; Yutaka Nakaya; Akira Takahashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-21
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  203     ISSN:  1537-6613     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-20     Completed Date:  2011-02-24     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  537-44     Citation Subset:  AIM; IM    
Affiliation:
Department of Preventive Environment and Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells
Humans
Interleukin-8 / secretion*
Mitogen-Activated Protein Kinases / metabolism*
Phosphorylation
Signal Transduction*
Vibrio parahaemolyticus / immunology*,  pathogenicity*
Viral Proteins / immunology*
Virulence Factors / immunology*
Chemical
Reg. No./Substance:
0/Interleukin-8; 0/Viral Proteins; 0/Virulence Factors; EC 2.7.11.24/Mitogen-Activated Protein Kinases
Comments/Corrections

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