| Vibrio fischeri flavohaemoglobin protects against nitric oxide during initiation of the squid-Vibrio symbiosis. | |
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MedLine Citation:
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PMID: 20815823 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nitric oxide (NO) is implicated in a wide range of biological processes, including innate immunity against pathogens, signal transduction and protection against oxidative stress. However, its possible roles in beneficial host-microbe associations are less well recognized. During the early stages of the squid-vibrio symbiosis, the bacterial symbiont Vibrio fischeri encounters host-derived NO, which has been hypothesized to serve as a specificity determinant. We demonstrate here that the flavohaemoglobin, Hmp, of V. fischeri protects against NO, both in culture and during colonization of the squid host. Transcriptional analyses indicate that hmp expression is highly responsive to NO, principally through the repressor, NsrR. Hmp protects V. fischeri from NO inhibition of aerobic respiration, and removes NO under both oxic and anoxic conditions. A Δhmp mutant of V. fischeri initiates squid colonization less effectively than wild type, but is rescued by the presence of an NO synthase inhibitor. The hmp promoter is activated during the initial stage of colonization, during which the Δhmp strain fails to form normal-sized aggregates of colonizing cells. Taken together, these results suggest that the sensing of host-derived NO by NsrR, and the subsequent removal of NO by Hmp, influence aggregate size and, thereby, V. fischeri colonization efficiency. |
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Authors:
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Yanling Wang; Anne K Dunn; Jacqueline Wilneff; Margaret J McFall-Ngai; Stephen Spiro; Edward G Ruby |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-29 |
Journal Detail:
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Title: Molecular microbiology Volume: 78 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-10 Completed Date: 2011-02-22 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 903-15 Citation Subset: IM |
Copyright Information:
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© 2010 Blackwell Publishing Ltd. |
Affiliation:
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Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Anaerobiosis Animals Bacterial Proteins / metabolism* Decapodiformes / immunology*, microbiology* Gene Expression Profiling Hemeproteins / metabolism* Nitric Oxide / metabolism*, toxicity Repressor Proteins / metabolism Symbiosis* Vibrio fischeri / immunology*, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 RR 12294/RR/NCRR NIH HHS; R01 RR012294-15/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Hemeproteins; 0/Repressor Proteins; 0/flavohemoprotein, Bacteria; 10102-43-9/Nitric Oxide |
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