| Vibrational stark effect probes for nucleic acids. | |
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MedLine Citation:
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PMID: 17877390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The vibrational Stark effect (VSE) has proven to be an effective method for the study of electric fields in proteins via the use of infrared probes. To explore the use of VSE in nucleic acids, we investigated the Stark spectroscopy of nine structurally diverse nucleosides. These nucleosides contained nitrile or azide probes in positions that correspond to both the major and minor grooves of DNA. The nitrile probes showed better characteristics and exhibited absorption frequencies over a broad range; that is, from 2253 cm-1 for 2'-O-cyanoethyl ribonucleosides 8 and 9 to 2102 cm(-1) for a 13C-labeled 5-thiocyanatomethyl-2'-deoxyuridine 3c. The largest Stark tuning rate observed was |Deltamu| = 1.1 cm(-1)/(MV/cm) for both 5-cyano-2'-deoxyuridine 1 and N2-nitrile-2'-deoxyguanosine 7. The latter is a particularly attractive probe because of its high extinction coefficient (epsilon = 412 M-1cm-1) and ease of incorporation into oligomers. |
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Authors:
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Lisa N Silverman; Michael E Pitzer; Peter O Ankomah; Steven G Boxer; Edward E Fenlon |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-09-18 |
Journal Detail:
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Title: The journal of physical chemistry. B Volume: 111 ISSN: 1520-6106 ISO Abbreviation: J Phys Chem B Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-04 Completed Date: 2007-11-28 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 101157530 Medline TA: J Phys Chem B Country: United States |
Other Details:
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Languages: eng Pagination: 11611-3 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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chemistry* Molecular Probe Techniques* Molecular Probes* Nitriles Nucleosides RNA / chemistry* Static Electricity |
| Grant Support | |
ID/Acronym/Agency:
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GM27738/GM/NIGMS NIH HHS; R01 GM027738-28/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Molecular Probes; 0/Nitriles; 0/Nucleosides; 63231-63-0/RNA; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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