| Vibrational Raman optical activity of proteins, nucleic acids, and viruses. | |
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MedLine Citation:
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PMID: 12606225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Due to its sensitivity to chirality, Raman optical activity (ROA), which may be measured as a small difference in vibrational Raman scattering from chiral molecules in right- and left-circularly polarized incident light, is a powerful probe of biomolecular structure in solution. Protein ROA spectra provide information on the secondary and tertiary structures of the polypeptide backbone, hydration, side-chain conformation, and structural elements present in denatured states. Nucleic acid ROA spectra yield information on the sugar ring conformation, the base stacking arrangement, and the mutual orientation of the sugar and base rings around the C-N glycosidic linkage. ROA is able to simultaneously probe the structures of both the protein and the nucleic acid components of intact viruses. This article gives a brief account of the theory and measurement of ROA and presents the ROA spectra of a selection of proteins, nucleic acids, and viruses which illustrate the applications of ROA spectroscopy in biomolecular research. |
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Authors:
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Ewan W Blanch; Lutz Hecht; Laurence D Barron |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: 29 ISSN: 1046-2023 ISO Abbreviation: Methods Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-27 Completed Date: 2003-09-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: United States |
Other Details:
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Languages: eng Pagination: 196-209 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Nucleic Acid Conformation Nucleic Acids / analysis, chemistry* Optics and Photonics Protein Folding Proteins / analysis, chemistry* Sensitivity and Specificity Spectrum Analysis, Raman / instrumentation, methods* Vibration Viruses / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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