Document Detail


Viable fibroblast matrix patch induces angiogenesis and increases myocardial blood flow in heart failure after myocardial infarction.
MedLine Citation:
PMID:  20486785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: This study examines a viable biodegradable three-dimensional fibroblast construct (3DFC) in a model of chronic heart failure. The viable fibroblasts, cultured on a vicryl mesh, secrete growth factors that stimulate angiogenesis.
METHODS: We ligated the left coronary artery of male Sprague-Dawley rats, implanted the 3DFC 3 weeks after myocardial infarction and obtained end point data 3 weeks later, that is, 6 weeks after myocardial infarction.
RESULTS: Implanting the 3DFC increases (p<0.05) myocardial blood flow twofold, microvessel formation (0.02±0.01 vs. 0.07±0.03 vessels/μm2), and ventricular wall thickness (0.53±0.02 to 1.02±0.17mm). The 3DFC shifts the passive pressure volume loop toward the pressure axis but does not alter left ventricular (LV) ejection fraction, systolic displacement, LV end-diastolic pressure/dimension, or LV cavity area. The 3DFC stimulates selected cytokine activation with a decrease in the proinflammatory cascade and increased total protein content stimulated by strained 3DFC in vitro.
CONCLUSION: The 3DFC functions as a cell delivery device providing matrix support for resident cell survival and integration into the heart. The imbedded fibroblasts of the 3DFC release a complex blend of cardioactive cytokines promoting increases in microvessel density and anterior wall blood flow but does not improve ejection fraction or alter LV remodeling.
Authors:
Jordan Lancaster; Elizabeth Juneman; Tracy Hagerty; Rose Do; Michael Hicks; Kate Meltzer; Paul Standley; Mohamed Gaballa; Robert Kellar; Steven Goldman; Hoang Thai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  16     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3065-73     Citation Subset:  IM    
Affiliation:
Department of Cardiology, Southern Arizona VA HealthCare System, Tucson, Arizona 85723, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics
Coronary Circulation / physiology
Cytokines / metabolism
Echocardiography
Fibroblasts / cytology*,  metabolism*
Heart Failure / therapy*
Male
Myocardial Infarction / therapy*
Neovascularization, Physiologic / physiology
Rats
Rats, Sprague-Dawley
Tissue Engineering / methods*
Chemical
Reg. No./Substance:
0/Cytokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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