| Viable fibroblast matrix patch induces angiogenesis and increases myocardial blood flow in heart failure after myocardial infarction. | |
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MedLine Citation:
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PMID: 20486785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: This study examines a viable biodegradable three-dimensional fibroblast construct (3DFC) in a model of chronic heart failure. The viable fibroblasts, cultured on a vicryl mesh, secrete growth factors that stimulate angiogenesis. METHODS: We ligated the left coronary artery of male Sprague-Dawley rats, implanted the 3DFC 3 weeks after myocardial infarction and obtained end point data 3 weeks later, that is, 6 weeks after myocardial infarction. RESULTS: Implanting the 3DFC increases (p<0.05) myocardial blood flow twofold, microvessel formation (0.02±0.01 vs. 0.07±0.03 vessels/μm2), and ventricular wall thickness (0.53±0.02 to 1.02±0.17mm). The 3DFC shifts the passive pressure volume loop toward the pressure axis but does not alter left ventricular (LV) ejection fraction, systolic displacement, LV end-diastolic pressure/dimension, or LV cavity area. The 3DFC stimulates selected cytokine activation with a decrease in the proinflammatory cascade and increased total protein content stimulated by strained 3DFC in vitro. CONCLUSION: The 3DFC functions as a cell delivery device providing matrix support for resident cell survival and integration into the heart. The imbedded fibroblasts of the 3DFC release a complex blend of cardioactive cytokines promoting increases in microvessel density and anterior wall blood flow but does not improve ejection fraction or alter LV remodeling. |
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Authors:
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Jordan Lancaster; Elizabeth Juneman; Tracy Hagerty; Rose Do; Michael Hicks; Kate Meltzer; Paul Standley; Mohamed Gaballa; Robert Kellar; Steven Goldman; Hoang Thai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 16 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 3065-73 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Southern Arizona VA HealthCare System, Tucson, Arizona 85723, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomechanics Coronary Circulation / physiology Cytokines / metabolism Echocardiography Fibroblasts / cytology*, metabolism* Heart Failure / therapy* Male Myocardial Infarction / therapy* Neovascularization, Physiologic / physiology Rats Rats, Sprague-Dawley Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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