Document Detail

Viable biobanking of primary head and neck squamous cell carcinoma.
MedLine Citation:
PMID:  23299699     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES/HYPOTHESIS: To determine the feasibility of viable storage of head and neck squamous cell carcinoma (HNSCC) for regrowth of cells in culture.
STUDY DESIGN: Laboratory-based translational study.
METHODS: Methods for intermediate-term frozen storage of viable HNSCC were explored using small pieces of primary tumor and dissociated HNSCC cells after short-term culture. Viable cells after freezing were confirmed by adherence to tissue culture plates, cell morphology, and increased cell or colony density. Two cultures were immunostained for cytokeratin to confirm epithelial origin of viable cultured cells after freezing.
RESULTS: Six primary HNSCCs (two oral cavity, three larynx, one oropharynx) and two HNSCCs that had been passaged through a xenograft (two oral cavity) were dissociated to single cells and grown in short-term cell culture for 0 to 12 passages. After short-term culture, cells were frozen for up to 8 months, thawed, and replated. Frozen cells derived from all tumors (six primary and two xenografts) were successfully replated with cultures lasting >7 days with seven of eight tumors presenting increased colony or cell density over 1 week of growth after freezing. In total, 15 of 15 tested samples derived from six primary and two xenografted HNSCCs were viable after freezing.
CONCLUSIONS: In the current study, we show that biopreservation of primary or xenografted HNSCC using short-term cell culture is feasible. Initial short-term cell culture was required for successful storage and viability of frozen cells. These proof-of-principle studies, if more widely implemented, could improve preclinical testing of new therapies for HNSCC.
Jose M Godoy; Andrew Sewell; Benjamin Johnston; Brandee T Brown; Xinyuan Lu; Robert J Sinard; Sarah Rohde; Kyle Mannion; James L Netterville; Wendell G Yarbrough
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-08
Journal Detail:
Title:  The Laryngoscope     Volume:  123     ISSN:  1531-4995     ISO Abbreviation:  Laryngoscope     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-05-28     Revised Date:  2014-01-17    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  United States    
Other Details:
Languages:  eng     Pagination:  641-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 The American Laryngological, Rhinological, and Otological Society, Inc.
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MeSH Terms
Carcinoma, Squamous Cell*
Feasibility Studies
Head and Neck Neoplasms*
Keratins / metabolism
Laryngeal Neoplasms
Mouth Neoplasms
Oropharyngeal Neoplasms
Tissue Banks
Transplantation, Heterologous
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:

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