Document Detail


Viable fertile mice generated from fully pluripotent iPS cells derived from adult somatic cells.
MedLine Citation:
PMID:  20549390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic origin. Achieving fully pluripotent iPS cells from multiple cell types, especially easily accessible adult tissues, will lead to a much greater clinical impact. We successfully generated high-pluripotency iPS cells from adult tail tip fibroblasts (TTF) that resulted in viable, full-term, fertile TTF-iPS animals with no obvious teratoma formation or other developmental abnormalities. Comparison of iPS cells from embryonic origin (MEF), progenitor cells (neural stem cells) or differentiated somatic cells (TTF) reveals that fully pluripotent developmental potential can be reached by each cell type, although with different induction efficiencies. This work provides the means for studying the mechanisms and regulation of direct reprogramming, and has encouraging implications for future clinical applications and therapeutic interventions.
Authors:
Xiao-yang Zhao; Wei Li; Zhuo Lv; Lei Liu; Man Tong; Tang Hai; Jie Hao; Xiang Wang; Liu Wang; Fanyi Zeng; Qi Zhou
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Stem cell reviews     Volume:  6     ISSN:  1558-6804     ISO Abbreviation:  Stem Cell Rev     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-20     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101255952     Medline TA:  Stem Cell Rev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  390-7     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Adult Stem Cells / physiology
Animals
Cell Differentiation* / physiology
Cloning, Organism / methods*
Fertility / physiology
Fetal Viability / physiology
Fibroblasts / physiology*
Induced Pluripotent Stem Cells / physiology*
Mice
Mice, Transgenic / physiology
Models, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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