Document Detail

Viability of some metabolic processes in the isolated toad brain adapted to two osmotic environments.
MedLine Citation:
PMID:  1185179     Owner:  NLM     Status:  MEDLINE    
The viability of the isolated toad brain in an aerated Ringer-like medium has been evaluated by the following criteria: 1) amino acid content before and after incubation; 2) accumulation of amino acids in the incubation medium; 3) a comparison of glucose utilization and [U-14C]glucose metabolism with that occurring in vivo; 4) tissue swelling; and 5) tissue lactate contents. On the basis of these criteria, the isolated toad brain, from toads adapted to a fresh-water or a salt-water environment, retains considerable metabolic integrity for at least 2 hr of incubation at 25 degrees C. Specifically, there was no swelling of the tissue, no apparent accumulation of lactate in the tissue, glucose appeared to be utilized at a rate not too different from that calculated for the toad brain in vivo, and the distribution of label from [U-14C]glucose had an overall pattern which resembled that observed in vivo. The tissue levels of amino acids were generally stable in vitro; however, there was a marked decline in the content of aspartate. The accumulation of amino acids in the medium varied considerably from one amino acid to another. Thus, there was very little net efflux of aspartate, GABA, and glutamate from the tissue but considerable net efflux of glutamine. This efflux of amino acids was greater from brains of hyperosmotically adapted toads than from the brains of toads adapted to fresh water by amounts proportional to their initial tissue contents.
R P Shank; J T Whiten; C F Baxter
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurobiology     Volume:  6     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1976-01-23     Completed Date:  1976-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  145-57     Citation Subset:  IM    
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MeSH Terms
Adaptation, Physiological
Amino Acids / metabolism*
Brain / metabolism*
Glucose / metabolism*
Osmolar Concentration
Reg. No./Substance:
0/Amino Acids; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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