Document Detail


Viability of preadipocytes in vitro: the influence of local anesthetics and pH.
MedLine Citation:
PMID:  19438665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Autogenous fat transfer with lipoinjection for soft tissue augmentation is a commonly used surgical technique. Abundant donor tissue availability and relative ease of harvesting have made autologous fat an attractive soft tissue filler. The overall reliability of this technique is often disputed, and different authors describe different results after autologous fat transplantation despite using similar techniques. In this study, we examined the influence of different local anesthetics commonly used in fat harvest and the pH of the anesthetic solution on the viability of harvested preadipocytes. METHODS AND MATERIALS: Preadipocytes were incubated with 1% lidocaine, 1% articaine plus epinephrine 1:200,000, 0.75% ropivacaine, and 1% prilocaine or our standardized tumescent solution (1 L of 0.9% sodium chloride solution plus 25 mL of 1% articaine plus epinephrine 1:200,000 plus 25 mL of bicarbonate) for 30 minutes. Additionally, we incubated cells with the local anesthetics as described above but diluted 1:2 with phosphate buffered saline (pH 7.4). Viability was measured using trypan blue dying as well as propidium iodine staining and fluorescence-activated cell sorting analysis. RESULTS: There are significant differences in the viability of preadipocytes under the influence of various local anesthetics. DISCUSSION: Our data could partially explain the varying results after autogenous fat transfer.
Authors:
Maike Keck; Juergen Janke; Klaus Ueberreiter
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Publication Detail:
Type:  Journal Article     Date:  2009-05-12
Journal Detail:
Title:  Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]     Volume:  35     ISSN:  1524-4725     ISO Abbreviation:  Dermatol Surg     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504371     Medline TA:  Dermatol Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1251-7     Citation Subset:  IM    
Affiliation:
Department of Plastic and Aesthetic Surgery, Asklepios Clinic Birkenwerder, Birkenwerder, Germany. maike.keck@meduniwien.ac.at
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / drug effects,  physiology*
Amides / pharmacology
Anesthetics, Local / pharmacology*
Carticaine / pharmacology
Cell Survival
Cells, Cultured
Epinephrine / pharmacology
Female
Humans
Hydrogen-Ion Concentration
Lidocaine / pharmacology
Male
Prilocaine / pharmacology
Tissue and Organ Harvesting
Chemical
Reg. No./Substance:
0/Amides; 0/Anesthetics, Local; 137-58-6/Lidocaine; 23964-58-1/Carticaine; 51-43-4/Epinephrine; 721-50-6/Prilocaine; 84057-95-4/ropivacaine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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