Document Detail


Viability of nuclei of two-cell mouse embryos stored at 4 degrees C and fused with blastomeres of fresh two-cell embryos.
MedLine Citation:
PMID:  1395687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study compares the resistance of the nuclei and the cytoplasm of two-cell mouse embryos to short-term storage at low temperature above 0 degrees C. Two-cell embryos were stored at 4 degrees C for 24-96 h in PB1 containing 0.25, 0.5, 0.75, and 1.0 M sucrose. The development to blastocysts in culture was highest in the presence of 0.5 M sucrose. However, only 3% of the embryos developed into blastocysts after 96 h of storage. On the other hand, the viability of the nuclei of two-cell embryos stored at 4 degrees C was significantly prolonged when they were transplanted into a blastomere of enucleated fresh F1 (C57BL/6JXCBA) two-cell embryos. The proportions of chimeric embryos that developed to blastocysts were 88, 67, 76, 71, 64, 45, 32, and 20% following storage for 0, 48, 72, 96, 120, 144, 168, and 192 h, respectively. In addition, there was no difference in the coat color of the young derived from nuclei stored at 4 degrees C or fresh nuclei, although the proportions of chimeric embryos that developed into live young after transfer tended to decrease with increased storage time. Moreover, the viability of nuclei stored at 4 degrees C for 192 h was confirmed in the germ cell population of chimeric mice mated with albino mice. These results demonstrated that the nuclei in the two-cell mouse embryos were more resistant to storage at low temperature than the cytoplasm.
Authors:
K Nakamura; Y Tsunoda
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  29     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-11-13     Completed Date:  1992-11-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  493-9     Citation Subset:  IM    
Affiliation:
Safety Evaluation Laboratory Kaken Pharmaceutical Co., Ltd., Shizuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastomeres / ultrastructure
Cell Fusion
Cell Nucleus / ultrastructure
Cell Survival
Chimera
Cryopreservation*
Cytoplasm / ultrastructure
Embryo Transfer
Embryo, Mammalian* / ultrastructure
Embryonic and Fetal Development
Female
Male
Mice
Nuclear Transfer Techniques
Pregnancy
Temperature

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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