Document Detail

Viability of engineered vessels as arterial substitutes.
MedLine Citation:
PMID:  18346580     Owner:  NLM     Status:  MEDLINE    
The search for vessel substitutes to replace small-/medium-caliber vessels is an ongoing concern for vascular surgeons. Engineered vessels were designed for use as arterial equivalents and assessed in an in vivo model in dog. Three study groups were established: clinical expanded polytetrafluoroethylene (ePTFE; control, n = 24), ePTFE seeding with endothelial cells (EC graft, n = 12), and ePTFE with a fibroblast matrix seeded with EC (FM+EC graft, n = 12). Grafts were subjected to a custom-designed femoral ex vivo circuit and implanted in the carotid artery for 60 days. The viability of the prosthetic grafts was evaluated. The ex vivo circuit revealed that the presence of a fibroblast matrix induced over double the cell retention compared to EC grafts. A significant reduction in platelet adhesion in EC grafts was observed. After their in vivo implantation, the engineered vessels were more efficient at avoiding occlusion than the prosthetic grafts. The FM+EC grafts induced more endothelialization than those seeded with ECs alone. Intimal hyperplasia response was reduced in the EC substitutes. Significant differences in apoptotic cells emerged between the EC and control ePTFE grafts. In conclusion, engineered vessels showed improved initial patency over ePTFE grafts. The EC graft was best at combating restenosis, a good indicator of the long-term efficiency of the graft.
Natalio García-Honduvilla; Belén Domínguez; Gemma Pascual; Cristina Escudero; Francisco Minguela; Juan Manuel Bellón; Julia Buján
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of vascular surgery     Volume:  22     ISSN:  0890-5096     ISO Abbreviation:  Ann Vasc Surg     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-18     Completed Date:  2008-07-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703941     Medline TA:  Ann Vasc Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  255-65     Citation Subset:  IM    
Department of Surgery, Faculty of Medicine, University of Alcalá, Alcalá de Henares, and Networking Research Center on Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
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MeSH Terms
Blood Vessel Prosthesis*
Carotid Arteries / surgery*
Collagen Type I / analysis
Endothelium, Vascular / cytology
Fibroblasts / cytology
Graft Survival
Microscopy, Electron, Scanning
Platelet Adhesiveness
Tissue Engineering*
Tunica Intima / pathology
Reg. No./Substance:
0/Collagen Type I; 9002-84-0/Polytetrafluoroethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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