Document Detail


Viability and endogenous substrates used during starvation survival of Rhodospirillum rubrum.
MedLine Citation:
PMID:  96087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells of Rhodospirillum rubrum were grown photoorganotrophically and chemoorganotrophically and then starved for organic carbon and combined nitrogen under four conditions: anaerobically in the light and dark and aerobically in the light and dark. Illumination prolonged viability and suppressed the net degradation of cell material of phototrophically grown cells, but had no effect on chemotrophically grown cells that did not contain bacteriochlorophyll. The half-life survival times of carbohydrate-rich phototrophically grown cells during starvation anaerobically or aerobically in the light were 17 and 14.5 days, respectively. The values for starvation aerobically and anaerobically in the dark were 3 and 0.5 days, respectively. Chemotrophically grown cells had half-life survival times of 3 and 4 days during starvation aerobically in the light and dark, respectively, and 0.8 day during starvation anaerobically in the light or dark. Of all cell constituents examined, carbohydrate was most extensively degraded during starvation, although the rate of degradation was slowest for phototrophically grown cells starved anaerobically in the light. Phototrophically grown cells containing poly-beta-hydroxybutyrate as carbon reserve were less able to survive starvation anaerobically in the light than were carbohydrate-rich cells starved under comparable conditions. Light intensity had a significant effect on viability of phototrophically grown cells starving anaerobically. At light intensities of 320 to 650 lx, the half-life survival times were 17 to 24 days. At 2,950 to 10,500 lx, the survival times decreased to 1.5 to 5.5 days. The kinetics of cell death correlated well with the rate of loss of cell mass of starving cells. However, the cause of death could not be attributed to degradation of any specific cell component.
Authors:
J A Breznak; C J Potrikus; N Pfennig; J C Ensign
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  134     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1978 May 
Date Detail:
Created Date:  1978-08-14     Completed Date:  1978-08-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Anaerobiosis
Bacterial Proteins / metabolism*
Carbohydrate Metabolism*
DNA, Bacterial / metabolism*
Half-Life
Light
RNA, Bacterial / metabolism*
Rhodospirillum rubrum / metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/RNA, Bacterial
Comments/Corrections

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