Document Detail

Viability of cells in cryopreserved canine cardiovascular organs for transplantation.
MedLine Citation:
PMID:  11079614     Owner:  NLM     Status:  MEDLINE    
To determine applicability of the cryopreservation procedure for vessel grafts, the viability of endothelial cells (ECs) among the whole cells in three kinds of organs artery, vein, trachea in mongrel dogs was evaluated on the basis of histological analysis. The Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GSA-FITC) and propidium iodide (PI) double staining methods were combined with flow cytometry (FCM), which was able to simultaneously determine the viability of whole cells and ECs from the same tissue, were performed after harvesting, after antibiotic solution treatment, and after cryopreservation and thawing. In most cases, the viability of ECs is lower than that of whole cells from veins and arteries. The viability of whole cells in veins was maintained until the antibiotic solution treatment and then decreased significantly after cryopreservation and thawing, while the ECs began to decrease significantly after the antibiotic solution treatment and more markedly decreased after thawing. The viability of ECs and whole cells from arteries was similar to that of the veins' conditions. The viability of whole cells from the trachea decreased with a similar pattern to that of the ECs from vessels. In consideration of maintaining cell viability among the three kinds of organs, the viability of arteries was better than that of the others. The cells in the trachea demonstrated a lower viability than the vessels. The effect of antibiotic solution treatment on the reduction of cell viability depends on the treatment time and temperature.
J C Park; H J Sung; D H Lee; Y H Park; B K Cho; H Suh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yonsei medical journal     Volume:  41     ISSN:  0513-5796     ISO Abbreviation:  Yonsei Med. J.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-20     Completed Date:  2000-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0414003     Medline TA:  Yonsei Med J     Country:  KOREA (SOUTH)    
Other Details:
Languages:  eng     Pagination:  556-62     Citation Subset:  IM    
Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
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MeSH Terms
Arteries / transplantation
Cell Survival
Coronary Vessels* / transplantation
Endothelium, Vascular / cytology,  physiology*
Trachea* / transplantation
Veins / transplantation

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