Document Detail


Viability assessment of primo-node slices from organ surface primo-vascular tissues in rats.
MedLine Citation:
PMID:  21185538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primo-vascular system is a novel thread-like structure which is recently rediscovered, but its cellular properties are largely unknown. In this study, a slice preparation for primo-nodes was developed to facilitate study of the cellular properties of primo-node cells in vitro. Slices (4-8 slices; 200 μm thick) were sectioned from single primo-nodes collected from the abdominal organ surface of rats and incubated in oxygenated Krebs solution at 25°C or 31°C for up to 7 hours. Trypan blue staining and whole-cell patch-clamp recordings were performed to estimate the viability of cells in the slices. Viability was largely maintained during the first 3 hours, but subsequently decreased (from 80% to 21%, p < 0.001). In addition, the viability of slices incubated at 31°C was higher than those incubated at 25°C (80%vs. 47%, p < 0.001). In whole-cell patch-clamp experiments, high resistance seals readily formed and primo-node cells showed a mean resting membrane potential (-38 mV) comparable to that recorded with sharp electrodes and outwardly-rectifying current-voltage relationships. The results show that the primo-node slices developed in this study maintained viability for up to 4 hours in vitro.
Authors:
Tae Hee Han; Chae Jeong Lim; Jae-Hong Choi; So Yeong Lee; Pan Dong Ryu
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of acupuncture and meridian studies     Volume:  3     ISSN:  2093-8152     ISO Abbreviation:  J Acupunct Meridian Stud     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-27     Completed Date:  2011-04-04     Revised Date:  2012-08-08    
Medline Journal Info:
Nlm Unique ID:  101490763     Medline TA:  J Acupunct Meridian Stud     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Korean Pharmacopuncture Institute. Published by .. All rights reserved.
Affiliation:
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Acupuncture Points*
Animal Structures / anatomy & histology,  chemistry,  physiology*
Animals
Histocytological Preparation Techniques / methods*
Male
Membrane Potentials
Microtomy
Rats
Rats, Sprague-Dawley
Staining and Labeling

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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