Document Detail

Viability assessment of primo-node slices from organ surface primo-vascular tissues in rats.
MedLine Citation:
PMID:  21185538     Owner:  NLM     Status:  MEDLINE    
The primo-vascular system is a novel thread-like structure which is recently rediscovered, but its cellular properties are largely unknown. In this study, a slice preparation for primo-nodes was developed to facilitate study of the cellular properties of primo-node cells in vitro. Slices (4-8 slices; 200 μm thick) were sectioned from single primo-nodes collected from the abdominal organ surface of rats and incubated in oxygenated Krebs solution at 25°C or 31°C for up to 7 hours. Trypan blue staining and whole-cell patch-clamp recordings were performed to estimate the viability of cells in the slices. Viability was largely maintained during the first 3 hours, but subsequently decreased (from 80% to 21%, p < 0.001). In addition, the viability of slices incubated at 31°C was higher than those incubated at 25°C (80%vs. 47%, p < 0.001). In whole-cell patch-clamp experiments, high resistance seals readily formed and primo-node cells showed a mean resting membrane potential (-38 mV) comparable to that recorded with sharp electrodes and outwardly-rectifying current-voltage relationships. The results show that the primo-node slices developed in this study maintained viability for up to 4 hours in vitro.
Tae Hee Han; Chae Jeong Lim; Jae-Hong Choi; So Yeong Lee; Pan Dong Ryu
Related Documents :
19067748 - Toward minimal bacterial cells: evolution vs. design.
16941448 - Bio-electrosprays: the next generation of electrified jets.
7937488 - Survivors of bursal b cell production and emigration.
19299118 - Engineered luciferases for molecular sensing in living cells.
1739078 - Expression of differentiation antigens and growth-related genes in normal kidney, autos...
16956748 - Identification of novel cell-wall active antifungal compounds.
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of acupuncture and meridian studies     Volume:  3     ISSN:  2093-8152     ISO Abbreviation:  J Acupunct Meridian Stud     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-27     Completed Date:  2011-04-04     Revised Date:  2012-08-08    
Medline Journal Info:
Nlm Unique ID:  101490763     Medline TA:  J Acupunct Meridian Stud     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Korean Pharmacopuncture Institute. Published by .. All rights reserved.
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acupuncture Points*
Animal Structures / anatomy & histology,  chemistry,  physiology*
Histocytological Preparation Techniques / methods*
Membrane Potentials
Rats, Sprague-Dawley
Staining and Labeling

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Putative primo-vascular system in mesentery of rats.
Next Document:  Meridian electrical potential response to acupuncture stimulation between operator and subject.