Document Detail


Viability and DNA damage responses of TPPII-deficient Myc- and Ras-transformed fibroblasts.
MedLine Citation:
PMID:  19539606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tripeptidyl peptidase II (TPPII) is a giant cytosolic protease. Previous protease inhibitor, overexpression and siRNA studies suggested that TPPII is important for viability and proliferation of tumor cells, and for their ionizing radiation-induced DNA damage response. The possibility that TPPII could be targeted for tumor therapy prompted us to study its role in transformed cells following genetic TPPII deletion. We generated cell lines from primary fibroblasts having conditional (floxed) TPPII alleles, transformed them with both the c-myc and H-ras oncogenes, and deleted TPPII using retroviral self-deleting Cre recombinase. Clonally derived TPPIIflox/flox and TPPII-/- transformed fibroblasts showed no influences of TPPII expression on basal cell survival and proliferation, nor on radiation-induced p53 activation, p21 induction, cell cycle arrest, apoptosis, or clonogenic cell death. Thus, our results do not support a generally important role of TPPII for viability and proliferation of transformed cells or their p53-mediated DNA damage response.
Authors:
Chizuko Tsurumi; Elke Firat; Simone Gaedicke; Jisen Huai; Pankaj Kumar Mandal; Gabriele Niedermann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  386     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-07-21     Completed Date:  2009-08-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  563-8     Citation Subset:  IM    
Affiliation:
Department of Radiation Oncology, University Hospital Freiburg, Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aminopeptidases
Animals
Cell Survival / genetics
Cell Transformation, Neoplastic / genetics*,  pathology
DNA Damage / genetics*
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Fibroblasts / enzymology,  pathology
Genes, myc
Genes, ras
Mice
Mice, Knockout
Serine Endopeptidases / genetics,  physiology*
Chemical
Reg. No./Substance:
EC 3.4.11.-/Aminopeptidases; EC 3.4.14.-/Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; EC 3.4.14.10/tripeptidyl-peptidase 2; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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