| Vessel microport technique for applications in cerebrovascular research. | |
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MedLine Citation:
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PMID: 19115415 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cerebrovascular research suffers from a lack of reliable methods with which to deliver exogenous substances effectively into the central nervous system (CNS) of small experimental animals. Here we describe a novel vessel microport surgical technique for a variety of cerebrovascular applications that is reproducible and well tolerated in mice. The procedure is based on the insertion of a vessel microport into the external carotid artery for substance delivery into the CNS via the internal carotid artery. The method results in selective substance delivery into the ipsilateral hemisphere. Other novel aspects of this surgical technique include the ability to perform multiple injections, study of conscious mice well removed from surgery, and lack of occlusion of the common or internal carotid artery that allows carotid flow to be maintained. The feasibility of this technique has been validated by infusion of HIV Tat protein to induce permeability of the blood-brain barrier and by implantation of tumor cells to establish a brain metastasis model. Thus, the described vessel microport technique can be employed in a variety of cerebrovascular research applications. |
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Authors:
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Lei Chen; Karin R Swartz; Michal Toborek |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of neuroscience research Volume: 87 ISSN: 1097-4547 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-09 Completed Date: 2009-07-13 Revised Date: 2013-02-14 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
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Languages: eng Pagination: 1718-27 Citation Subset: IM |
Copyright Information:
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Copyright 2008 Wiley-Liss, Inc. |
Affiliation:
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Molecular Neuroscience and Vascular Biology Laboratory, Lexington, Kentucky 40536, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood-Brain Barrier / physiopathology Brain / blood supply, pathology, physiopathology Brain Neoplasms / physiopathology Carotid Artery, Internal / surgery* Cerebrovascular Circulation Disease Models, Animal Drug Delivery Systems / methods* Mice Mice, Inbred C57BL Microvessels / pathology Neoplasm Metastasis Soot tat Gene Products, Human Immunodeficiency Virus / administration & dosage |
| Grant Support | |
ID/Acronym/Agency:
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MH 072567/MH/NIMH NIH HHS; MH 63022/MH/NIMH NIH HHS; NS 39254/NS/NINDS NIH HHS; P42 ES 07380/ES/NIEHS NIH HHS; P42 ES007380-139005/ES/NIEHS NIH HHS; R01 CA133257-04/CA/NCI NIH HHS; R01 DA027569/DA/NIDA NIH HHS; R01 DA027569-03/DA/NIDA NIH HHS; R01 MH063022-05/MH/NIMH NIH HHS; R01 MH063022-07/MH/NIMH NIH HHS; R01 MH072567/MH/NIMH NIH HHS; R01 MH072567-04/MH/NIMH NIH HHS; R01 NS039254-05/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Soot; 0/tat Gene Products, Human Immunodeficiency Virus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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