Document Detail

Vesicular trafficking of hepatic apolipoprotein B100 and its maturation to very low-density lipoprotein particles; studies from cells and cell-free systems.
MedLine Citation:
PMID:  15177262     Owner:  NLM     Status:  MEDLINE    
A cell-free system was established to study the process by which apolipoprotein (apo)B100-containing lipoproteins exit the endoplasmic reticulum (ER). ApoB was found in COPII vesicles with physical properties distinct from those containing other secreted proteins. When lipid synthesis in rat hepatoma cells was stimulated by fatty acid addition, fully lipidated apoB-lipoproteins of very low-density lipoprotein density were absent from the vesicles, but instead formed in a post-ER compartment. These data suggest that the COPII machinery in cells of hepatic and intestinal origin has evolved to sequester secreted cargoes with unique properties compared with those in other tissues, and that final lipidation occurs after a protein quality-control checkpoint is passed in the ER.
Jeffrey L Brodsky; Viktoria Gusarova; Edward A Fisher
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Trends in cardiovascular medicine     Volume:  14     ISSN:  1050-1738     ISO Abbreviation:  Trends Cardiovasc. Med.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-06-04     Completed Date:  2004-10-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9108337     Medline TA:  Trends Cardiovasc Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-32     Citation Subset:  IM    
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
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MeSH Terms
Apolipoprotein B-100
Apolipoproteins B / metabolism*
Cell-Free System
Endoplasmic Reticulum / metabolism*
Hepatocytes / metabolism*
Lipoproteins, VLDL / metabolism*
Protein Transport
Secretory Vesicles / metabolism*
Grant Support
Reg. No./Substance:
0/Apolipoprotein B-100; 0/Apolipoproteins B; 0/Lipoproteins, VLDL

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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