Document Detail


Vesicular traffic and golgi apparatus dynamics during mammalian spermatogenesis: implications for acrosome architecture.
MedLine Citation:
PMID:  10859246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vesicular membrane trafficking during acrosome biogenesis in bull and rhesus monkey spermatogenesis differs from the somatic cell paradigm as imaged dynamically using the Golgi apparatus probes beta-COP, giantin, Golgin-97, and Golgin-95/GM130. In particular, sorting and delivery of proteins seemed less precise during spermatogenesis. In early stages of spermiogenesis, many Golgi resident proteins and specific acrosomal markers were present in the acrosome. Trafficking in both round and elongating spermatids was similar to what has been described for somatic cells, as judged by the kinetics of Golgi protein incorporation into endoplasmic reticulum-like structures after brefeldin A treatment. These Golgi components were retrieved from the acrosome at later stages of differentiation and were completely devoid of immature spermatozoa. Our data suggest that active anterograde and retrograde vesicular transport trafficking pathways, involving both beta-COP- and clathrin-coated vesicles, are involved in retrieving Golgi proteins missorted to the acrosome and in controlling the growth and shape of this organelle.
Authors:
R D Moreno; J Ramalho-Santos; P Sutovsky; E K Chan; G Schatten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  63     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-24     Completed Date:  2000-08-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  89-98     Citation Subset:  IM    
Affiliation:
Oregon Regional Primate Research Center, Beaverton, Oregon 97006, USA.
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MeSH Terms
Descriptor/Qualifier:
Acrosome Reaction / physiology*
Animals
Autoantigens / metabolism
Biological Transport
Brefeldin A / pharmacology
Cell Compartmentation
Cell Line
Cells, Cultured
Coated Vesicles / metabolism*
Coatomer Protein / metabolism
Endoplasmic Reticulum / metabolism
Fluorescent Dyes
Golgi Apparatus / metabolism*,  ultrastructure
Macaca mulatta
Male
Membrane Proteins / metabolism
Microscopy, Electron / methods
Proteins*
Spermatids / metabolism,  ultrastructure
Spermatogenesis / physiology*
Grant Support
ID/Acronym/Agency:
U54 HD 18185/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Autoantigens; 0/Coatomer Protein; 0/Fluorescent Dyes; 0/Golgin subfamily A member 2; 0/Membrane Proteins; 0/Proteins; 0/macrogolgin; 20350-15-6/Brefeldin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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